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Título

Microsatellite DNA capture from enriched libraries

Autor González, Elena G. ; Zardoya, Rafael
Fecha de publicación 15-mar-2013
EditorHumana Press
Citación Methods in Molecular Biology 1006: 67-87 (2013)
ResumenMicrosatellites are DNA sequences of tandem repeats of one to six nucleotides, which are highly polymorphic, and thus the molecular markers of choice in many kinship, population genetic, and conservation studies. There have been significant technical improvements since the early methods for microsatellite isolation were developed, and today the most common procedures take advantage of the hybrid capture methods of enriched-targeted microsatellite DNA. Furthermore, recent advents in sequencing technologies (i.e., next-generation sequencing, NGS) have fostered the mining of microsatellite markers in non-model organisms, affording a cost-effective way of obtaining a large amount of sequence data potentially useful for loci characterization. The rapid improvements of NGS platforms together with the increase in available microsatellite information open new avenues to the understanding of the evolutionary forces that shape genetic structuring in wild populations. Here, we provide detailed methodological procedures for microsatellite isolation based on the screening of GT microsatellite-enriched libraries, either by cloning and Sanger sequencing of positive clones or by direct NGS. Guides for designing new species-specific primers and basic genotyping are also given. © Springer Science+Business Media, LLC 2013.
URI http://hdl.handle.net/10261/123993
DOI10.1007/978-1-62703-389-3-5
Identificadoresdoi: 10.1007/978-1-62703-389-3-5
issn: 1064-3745
e-issn: 1940-6029
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