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dc.contributor.authorLourenço, Artur B.es_ES
dc.contributor.authorMuñoz-Jiménez, Celiaes_ES
dc.contributor.authorVenegas-Calerón, Mónicaes_ES
dc.contributor.authorArtal-Sanz, Martaes_ES
dc.date.accessioned2015-10-28T10:01:25Z-
dc.date.available2015-10-28T10:01:25Z-
dc.date.issued2015-11-
dc.identifier.citationBiochimica et Biophysica Acta - Bioenergetics 1847(11): 1457-1468 (2015)es_ES
dc.identifier.issn0005-2728-
dc.identifier.urihttp://hdl.handle.net/10261/123981-
dc.description12 páginas; 5 figuras; 4 tablas; 4 figuras.-- This article is part of a Special Issue entitled: Mitochondrial Dysfunction in Aging. Guest Editor: Aleksandra Trifunovic.-- Under a Creative Commons license.es_ES
dc.description.abstractThe mitochondrial prohibitin complex, composed of two proteins, PHB-1 and PHB-2, is a context-dependent modulator of longevity. Specifically, prohibitin deficiency shortens the lifespan of otherwise wild type worms, while it dramatically extends the lifespan under compromised metabolic conditions. This extremely intriguingly phenotype has been linked to alterations in mitochondrial function and in fat metabolism. However, the true function of the mitochondrial prohibitin complex remains elusive. Here, we used gas chromatography coupled to a flame ionization detector (GC/FID) and 1H NMR spectroscopy to gain molecular insights into the effect of prohibitin depletion on the Caenorhabditis elegans metabolome. We analysed the effect of prohibitin deficiency in two different developmental stages and under two different conditions, which result in opposing longevity phenotypes, namely wild type worms and daf-2(e1370) insulin signalling deficient mutants. Prohibitin depletion was shown to alter the fatty acid (GC/FID) and 1H NMR metabolic profiles of wild type animals both at the fourth larval stage of development (L4) and at the young adult (YA) stage, while being more pronounced at the later stage. Furthermore, wild type and the diapause mutant daf-2(e1370), either expressing or not prohibitin, were clearly distinguishable based on their metabolic profiles, revealing changes in fatty acid composition, as well as in carbohydrate and amino acid metabolism. Moreover, the metabolic data indicate that daf-2(e1370) mutants are more robust than the wild type animals to changes induced by prohibitin depletion. The impact of prohibitin depletion on the C. elegans metabolome will be discussed herein in the scope of its effect on longevity.es_ES
dc.description.sponsorshipThis work was funded by a grant from the European Research Council (ERC-2011-StG-281691) to M.A.S.es_ES
dc.description.sponsorshipOpen Access funded by European Research Council.-
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/281691-
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectProhibitines_ES
dc.subjectInsulin signallinges_ES
dc.subjectMitochondriaes_ES
dc.subjectC. eleganses_ES
dc.subjectLongevityes_ES
dc.subjectMetabolomicses_ES
dc.titleAnalysis of the effect of the mitochondrial prohibitin complex, a context-dependent modulator of longevity, on the C. elegans metabolomees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.bbabio.2015.06.003-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.bbabio.2015.06.003es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderEuropean Commission-
dc.relation.csices_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000781es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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