English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/123981
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Analysis of the effect of the mitochondrial prohibitin complex, a context-dependent modulator of longevity, on the C. elegans metabolome

AuthorsLourenço, Artur B.; Muñoz-Jiménez, Celia; Venegas-Calerón, Mónica ; Artal-Sanz, Marta
KeywordsProhibitin
Insulin signalling
Mitochondria
C. elegans
Longevity
Metabolomics
Issue DateNov-2015
PublisherElsevier
CitationBiochimica et Biophysica Acta - Bioenergetics 1847(11): 1457-1468 (2015)
AbstractThe mitochondrial prohibitin complex, composed of two proteins, PHB-1 and PHB-2, is a context-dependent modulator of longevity. Specifically, prohibitin deficiency shortens the lifespan of otherwise wild type worms, while it dramatically extends the lifespan under compromised metabolic conditions. This extremely intriguingly phenotype has been linked to alterations in mitochondrial function and in fat metabolism. However, the true function of the mitochondrial prohibitin complex remains elusive. Here, we used gas chromatography coupled to a flame ionization detector (GC/FID) and 1H NMR spectroscopy to gain molecular insights into the effect of prohibitin depletion on the Caenorhabditis elegans metabolome. We analysed the effect of prohibitin deficiency in two different developmental stages and under two different conditions, which result in opposing longevity phenotypes, namely wild type worms and daf-2(e1370) insulin signalling deficient mutants. Prohibitin depletion was shown to alter the fatty acid (GC/FID) and 1H NMR metabolic profiles of wild type animals both at the fourth larval stage of development (L4) and at the young adult (YA) stage, while being more pronounced at the later stage. Furthermore, wild type and the diapause mutant daf-2(e1370), either expressing or not prohibitin, were clearly distinguishable based on their metabolic profiles, revealing changes in fatty acid composition, as well as in carbohydrate and amino acid metabolism. Moreover, the metabolic data indicate that daf-2(e1370) mutants are more robust than the wild type animals to changes induced by prohibitin depletion. The impact of prohibitin depletion on the C. elegans metabolome will be discussed herein in the scope of its effect on longevity.
Description12 páginas; 5 figuras; 4 tablas; 4 figuras.-- This article is part of a Special Issue entitled: Mitochondrial Dysfunction in Aging. Guest Editor: Aleksandra Trifunovic.-- Under a Creative Commons license.
Publisher version (URL)http://dx.doi.org/10.1016/j.bbabio.2015.06.003
URIhttp://hdl.handle.net/10261/123981
DOI10.1016/j.bbabio.2015.06.003
ISSN0005-2728
Appears in Collections:(CABD) Artículos
(IG) Artículos
Files in This Item:
File Description SizeFormat 
BBA_2015_V1847_P1457.pdfArtículo principal1,5 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.