English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/123899
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorCubillo, Eva-
dc.contributor.authorDíaz-Lopez, Antonio-
dc.contributor.authorCuevas, Eva P.-
dc.contributor.authorMoreno-Bueno, Gema-
dc.contributor.authorPeinado, Héctor-
dc.contributor.authorMontes, Amalia-
dc.contributor.authorSantos, Vanesa-
dc.contributor.authorPortillo, Francisco-
dc.contributor.authorCano, Amparo-
dc.date.accessioned2015-10-27T10:27:14Z-
dc.date.available2015-10-27T10:27:14Z-
dc.date.issued2013-
dc.identifierdoi: 10.1371/journal.pone.0059948-
dc.identifierissn: 1932-6203-
dc.identifier.citationPLoS ONE 8(3): e59948 (2013)-
dc.identifier.urihttp://hdl.handle.net/10261/123899-
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License.-
dc.description.abstractE12/E47 proteins (encoded by E2A gene) are members of the class I basic helix-loop-helix (bHLH) transcription factors (also known as E proteins). E47 has been described as repressor of E-cadherin and inducer of epithelial-mesenchymal transition (EMT). We reported previously that EMT mediated by E47 in MDCK cells occurs with a concomitant overexpression of Id1 and Id3 proteins. Id proteins belong to class V of HLH factors that lack the basic domain; they dimerise with E proteins and prevent their DNA interaction, thus, acting as dominant negative of E proteins. Here, we show that E47 interacts with Id1 in E47 overexpressing MDCK cells that underwent a full EMT as well as in mesenchymal breast carcinoma and melanoma cell lines. By conducting chromatin immunoprecipitation assays we demonstrate that E47 binds directly to the endogenous E-cadherin promoter of mesenchymal MDCK-E47 cells in a complex devoid of Id1. Importantly, our data suggest that both E47 and Id1 are required to maintain the mesenchymal phenotype of MDCK-E47 cells. These data support the collaboration between E47 and Id1 in the maintenance of EMT by mechanisms independent of the dominant negative action of Id1 on E47 binding to E-cadherin promoter. Finally, the analysis of several N0 breast tumour series indicates that the expression of E47 and ID1 is significantly associated with the basal-like phenotype supporting the biological significance of the present findings.-
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Education and Science (SAF2007-63051; SAF2010-21143; Consolider Ingenio 2010 CDS07/00017) to A.C.; (SAF2007-63075 and SAF2010-20175) to G.M.B.; Comunidad de Madrid (S2010/BMD-2303) to A.C and G.M.B.-
dc.publisherPublic Library of Science-
dc.relationS2010/BMD-2303/RECARE-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleE47 and Id1 interplay in epithelial-mesenchymal transition-
dc.typeartículo-
dc.identifier.doi10.1371/journal.pone.0059948-
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0059948-
dc.date.updated2015-10-27T10:27:15Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Educación y Ciencia (España)-
dc.contributor.funderComunidad de Madrid-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
Epithelial-Mesenchymal.pdf1,72 MBAdobe PDFThumbnail
View/Open
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.