English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/123814
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Mitotic arrest down-regulates FLIP expression and sensitizes tumor cells to the extrinsic pathway of apoptosis

AuthorsLópez-Rivas, Abelardo ; Medema, René H.; Sánchez-Pérez, Tania
Issue Date15-Jul-2012
CitationGordon Research Conference on Cell Death (2012)
AbstractCell cycle deregulation is a feature of tumor cells. Most of the current therapeutic (A) MDA-MB231 (B) HeLa strategies are based on the perturbation of the cell cycle, especially during mitosis. “Antimitotic drugs” perturb the correct assembly of the mitotic spindle triggering the activation of the spindle assembly checkpoint (SAC), which results in a mitotic arrest and eventually, cell death. However, sometimes cells go out of mitosis by “slippage”, being one of the main mechanism of resistance to these treatments. We have already reported that microtubule poisons treatment sensitizes tumor cells to TRAIL- induced apoptosis and also that FLIP down-regulation induced by these treatments is the key event in this sensitization. In this study we demonstrate that both FLIP down-regulation and sensitization to TRAIL- induced apoptosis are induced by mitotic arrest independently of checkpoint activation. Moreover, although targeting mitotic exit has recently been proposed as a better strategy to kill tumor cells than conventional anti-mitotic drugs, we have observed that treatments based on combination of mitotic arrest-inducing regimes with TRAIL are much more effective against tumor cells, highlighting the potential therapeutic benefit of the use of this combination against tumor cells. Finally, we show that CDK1/Cyclin B complex, but no other mitotic kinases, seems to have a role in FLIP down-regulation, as FLIP levels inversely correlate with CDK1 activity in mitosis.
URIhttp://hdl.handle.net/10261/123814
Appears in Collections:(CABIMER) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
Mitotic arrest down-regulates_SánchezPérez.pdf1,63 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.