Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/123583
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Meseguer, Salvador | es_ES |
dc.contributor.author | Escamilla Honrubia, Juan Manuel | es_ES |
dc.contributor.author | Mudduluru, Giridhar | es_ES |
dc.contributor.author | Allgayer, Heike | es_ES |
dc.contributor.author | Barettino, Domingo | es_ES |
dc.date.accessioned | 2015-10-20T09:38:00Z | - |
dc.date.available | 2015-10-20T09:38:00Z | - |
dc.date.issued | 2010-11-11 | - |
dc.identifier.citation | VIII Reunión de la Red Valenciana de Genómica y Proteómica (2010) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/123583 | - |
dc.description | Póster original presentado en la VIII Reunión de la Red Valenciana de Genómica y Proteómica. Valencia, 11 de Noviembre de 2010. | es_ES |
dc.description.abstract | MicroRNAs (miRNAs) are an emerging class of non-coding endogenous RNAs involved in multiple cellular processes, including cell differentiation. Treatment with Retinoic Acid (RA) results in neural differentiation of neuroblastoma cells. We wanted to elucidate whether miRNAs contribute to the gene expression changes induced by RA in neuroblastoma cells, and whether miRNA regulation is involved in the transduction of the RA signal. We show here that RA treatment of SH-SY5Y neuroblastoma cells results in profound changes in the expression pattern of miRNAs. Up to 42 different miRNA species significantly changed their expression (26 upregulated and 16 downregulated). Among them, the closely related miR-10a and -10b showed the most prominent expression changes. Induction of miR-10a and -10b by RA also could be detected in LA-N-1 neuroblastoma cells. Loss of function experiments demonstrated that miR-10a and -10b are essential mediators of RA-induced neuroblastoma differentiation and of the associated changes in migration, invasion, and in vivo metastasis. In addition, we found that the SR-family splicing factor SFRS1 (SF2/ASF) is a target for miR-10a -and -10b in HeLa and SH-SY5Y neuroblastoma cells. We show here that changes in miR-10a and -10b expression levels may regulate SFRS1-dependent alternative splicing and translational functions. Taken together, our results give support to the idea that miRNA regulation plays a key role in RA-induced neuroblastoma cell differentiation. The discovery of SFRS1 as direct target of miR-10a and -10b support the emerging functional interaction between two post-transcriptional mechanisms, microRNAs and splicing, in the neuronal differentiation context. | es_ES |
dc.description.sponsorship | Funded by grants SAF2006-00647 and SAF2007-60780 and ACOMP 09/212. S.M. was the recipientof an EACR training and travel fellowship award, and a CSIC-I3P pre-doctoral fellowship/contract. | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Micro-RNAs-10a and -10b contribute to Retinoic Acid-induced differentiation of neuroblastoma cells and target the alternative splicing regulatory factor SFRS1 (SF2/ASF) | es_ES |
dc.type | póster de congreso | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | es_ES |
dc.relation.csic | Sí | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6670 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | póster de congreso | - |
Aparece en las colecciones: | (IBV) Comunicaciones congresos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
posterRVGP_2010.pdf | 3,28 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
245
checked on 23-abr-2024
Download(s)
427
checked on 23-abr-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.