Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/123546
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Multivalent glycosylation of fluorescent gold nanoclusters promotes increased human dendritic cell targeting via multiple endocytic pathways |
Autor: | Le Guével, Xavier; Pérez-Perrino, Mónica CSIC ORCID; Fernández, Tahia; Palomares, Francisca; Torres, María José; Blanca, Miguel; Rojo, Francisco Javier ; Mayorga, Cristobalina | Fecha de publicación: | 2015 | Citación: | ACS Applied Materials and Interfaces 7: 20945- 20956 (2015) | Resumen: | We report the synthesis and characterization of gold nanoclusters (Au NCs) stabilized by a mixture of zwitterionic and multivalent mannose ligands. Characterization of this carbohydrated nanosystem confirms its small size (∼2 nm), intense red-NIR fluorescence, relatively high affinity to lectin (ConA), and stability in physiological media. Cell studies performed using human-monocyte-derived dendritic cells (DCs) show that Au NC uptake efficiency is greatly enhanced by the presence of surface carbohydrate (>250% compared to noncarbohydrated Au NCs), allowing their detection in cells by fluorescence following incubation with concentrations as low as 1 μg mL<sup>-1</sup>. Investigation using electron microscopy and pharmacological inhibitors indicates that Au NC uptake is mediated by multiple endocytic pathways involving the engulfment of Au NCs into endosomes and partial transport to lysosomes. Results show that clathrin- and F-actin-dependent pathways play major roles in Au NC uptake by DCs, regardless of whether or not they are coated with carbohydrates. In contrast, a specific C-lectin inhibitor induces a 60% decrease in DC particle uptake only for the carbohydrate-coated Au NCs. This study demonstrates that the combination of ultrasmall gold NCs and functionalization with multivalent mannose ligands results in greatly enhanced human DC targeting, presumably due to increased diffusion and target cell binding, respectively. | URI: | http://hdl.handle.net/10261/123546 | DOI: | 10.1021/acsami.5b06541 | Identificadores: | doi: 10.1021/acsami.5b06541 issn: 1944-8252 |
Aparece en las colecciones: | (IIQ) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
ACS applie dMateriasl and interfaces revised.pdf | 5,72 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
58
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
53
checked on 19-feb-2024
Page view(s)
283
checked on 24-abr-2024
Download(s)
422
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.