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Title

Development of microsatellite markers as a molecular tool for conservation studies of the Mediterranean reef builder coral Cladocora caespitosa (Anthozoa, Scleractinia)

AuthorsCasado-Amezúa, P. ; García-Jiménez, Ricardo; Kersting, D. K.; Templado, José ; Coffroth, Mary Alice; Merino, Paula; Acevedo, Iván ; Machordom, Annie
KeywordsConservation genetics
Microsatellite markers
Endangered species
Scleractinia
Issue Date21-Jul-2011
PublisherOxford University Press
CitationJournal of Heredity 102(5): 622-626 (2011)
AbstractCladocora caespitosa is a reef-building zooxanthellate scleractinian coral in the Mediterranean Sea. Mortality events have recurrently affected this species during the last decade. Thus, knowledge of its genetic structure, population diversity, and connectivity is needed to accomplish suitable conservation plans. In order to obtain a better understanding of the population genetics of this species, 13 highly variable microsatellites markers were developed from a naturally bleached colony. The developed primers failed to amplify zooxanthella DNA, isolated from C. caespitosa, verifying that these markers were of the coral and not algal symbiont origin. The degree of polymorphism of these loci was tested on tissue samples from 28 colonies. The allele number for each loci ranged from 2 to 13 (mean Na = 5.4), with an average observed heterozygosity of 0.42 (He = 0.43) and all loci were in Hardy-Weinberg equilibrium. These new markers should be useful in future conservation genetic studies and will help to improve the resolution of the individual identification within this coral species. Primers were also tested in Oculina patagonica, with successful amplifications of several loci. The American Genetic Association. 2011. All rights reserved. For permissions, please email: journals. permissions@oup.com.2011 © The American Genetic Association. 2011. All rights reserved.
Description[EN] This adaptation text reproduces chapter II of the dissertation “Results”: Estudio genético de la estructura poblacional y conectividad de dos corales endémicos del Mediterráneo: Astroides calycularis (Pallas, 1766) y Cladocora caespitosa (Linnaeus, 1767)= Genetic assessment of population structure and connectivity in two endemic Mediterranean corals: Astroides calycularis (Pallas, 1766) and Cladocora caespitosa (Linnaeus, 1767), of P. Casado-Amezúa (2012). http://hdl.handle.net/10261/134913
[ES] Este artículo es una adaptación del capítulo II de “Resultados” de la tesis doctoral: Estudio genético de la estructura poblacional y conectividad de dos corales endémicos del Mediterráneo: Astroides calycularis (Pallas, 1766) y Cladocora caespitosa (Linnaeus, 1767)= Genetic assessment of population structure and connectivity in two endemic Mediterranean corals: Astroides calycularis (Pallas, 1766) and Cladocora caespitosa (Linnaeus, 1767), of P. Casado-Amezúa (2012). http://hdl.handle.net/10261/134913
Publisher version (URL)http://dx.doi.org/10.1093/jhered/esr070
URIhttp://hdl.handle.net/10261/123390
DOI10.1093/jhered/esr070
Identifiersdoi: 10.1093/jhered/esr070
issn: 0022-1503
e-issn: 1465-7333
Appears in Collections:(MNCN) Artículos
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