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Title

Altered activity and expression of cytosolic peptidases in colorectal cancer

AuthorsPérez, Itxaro; Blanco, Lorena; Sanz, Begoña; Errarte, Peio; Ariz, Usue; Beitia, Mader; Fernández, Ainhoa; Loizate, Alberto; Candenas, M. Luz ; Pinto Pérez, Francisco M. ; Gil, Javier; López, José Luis; Larrinaga, Gorka
Issue Date2015
PublisherIvyspring International Publisher
CitationInternational Journal of Medical Sciences 12: 458- 467 (2015)
Abstract© 2015 Ivyspring International Publisher. Background and Objective: The role of peptidases in carcinogenic processes and their potential usefulness as tumor markers in colorectal cancer (CRC) have been classically attributed to cell-surface enzymes. The objective of the present study was to analyze the activity and mRNA expression of three cytosolic peptidases in the CRC and to correlate the obtained results with classic histopathological parameters for tumor prognosis and survival. Methods: The activity and mRNA levels of puromycin-sensitive aminopeptidase (PSA), ami-nopeptidase B (APB) and pyroglutamyl-peptidase I (PGI) were measured by fluorimetric and quantitative RT-PCR methods in colorectal mucosa and tumor tissues and plasma samples from CRC patients (n=81). Results: 1) PSA and APB activity was higher in adenomas and carcinomas than in the uninvolved mucosa. 2) mRNA levels of PSA and PGI was lower in tumors. 3) PGI activity in CRC tissue correlated negatively with histological grade, tumor size and 5-year overall suvival of CRC patients. 4) Higher plasmatic APB activity was independently associated with better 5-year overall survival. Conclusions: Data suggest that cytosolic peptidases may be involved in colorectal carcinogenesis and point to the determination of this enzymes as a valuable method in the determination of CRC prognosis.
URIhttp://hdl.handle.net/10261/123271
DOI10.7150/ijms.11808
Identifiersdoi: 10.7150/ijms.11808
issn: 1449-1907
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