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dc.contributor.authorDíaz-Toledano, Rosaes_ES
dc.contributor.authorAriza-Mateos, Ascensiónes_ES
dc.contributor.authorBirk, A.es_ES
dc.contributor.authorMartínez-García, Belénes_ES
dc.contributor.authorGómez, Jordies_ES
dc.identifier.citationNucleic Acids Researches_ES
dc.descriptionSupplementari Data http://nar.oxfordjournals.org/content/suppl/2009/10/12/gkp553.DC1es_ES
dc.description.abstractIt has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed that this region switches to an open conformation triggered by the liver-specific microRNA, miR-122. This structural transition, observed in vitro, may be the mechanistic basis for the involvement of downstream IRES structural domain VI in translation, as well as providing a role of liver-specific miR-122 in HCV infection. In addition, the induced RNA switching at the 5′ untranslated region could ultimately represent a new mechanism of action of micro-RNAs.es_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación BIO2007-60106, BIO2004-06114 Proyecto Excelencia Comunidad Autónoma de Andalucía CVI-03050; FISS (CIBERehd); FIPSE 36549/06 for work in Granada; National Institutes of Health grant NIH/NIDA K01 DAO18262 for work in Doylestown, Pennsylvania. Funding for open access charge: BIO-2007, 60106 (IPB-LN) CSIC. MCyT.es_ES
dc.publisherOxford University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleIn vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNAes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.rights.licenseThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderJunta de Andalucíaes_ES
dc.contributor.funderCentro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España)es_ES
dc.contributor.funderFundación para la Investigación y la Prevención del Sida en Españaes_ES
dc.contributor.funderNational Institutes of Health (US)es_ES
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