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NAPPA as a real new method for protein microarray generation

AutorDíez, Paula; González González, María; Lourido, Lucía; Dégano, Rosa M.; Ibarrola, Nieves ; Casado-Vela, Juan; LaBaer, Joshua; Fuentes, Manuel
Palabras claveProtein microarray
NAPPA
High-throughput screening
Biomarker
Protein-protein interaction
Microarray generation
Fecha de publicación2015
EditorMultidisciplinary Digital Publishing Institute
CitaciónMicroarrays 4(2): 214-227 (2015)
ResumenNucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years.
DescripciónThis is an open access article distributed under the Creative Commons Attribution License.-- This article belongs to the Special Issue "New and Old Technologies for Generation of Microarrays".
Versión del editorhttp://dx.doi.org/10.3390/microarrays4020214
URIhttp://hdl.handle.net/10261/122794
DOI10.3390/microarrays4020214
ISSN2076-3905
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