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http://hdl.handle.net/10261/122793
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dc.contributor.author | Lachaud, Christian C. | - |
dc.contributor.author | Pezzolla, Daniela | - |
dc.contributor.author | Domínguez-Rodríguez, Alejandro | - |
dc.contributor.author | Smani, Tarik | - |
dc.contributor.author | Soria Escoms, Bernat | - |
dc.contributor.author | Hmadcha, Abdelkrim | - |
dc.date.accessioned | 2015-09-29T12:55:26Z | - |
dc.date.available | 2015-09-29T12:55:26Z | - |
dc.date.issued | 2013-02-06 | - |
dc.identifier | doi: 10.1371/journal.pone.0055181 | - |
dc.identifier | issn: 1932-6203 | - |
dc.identifier.citation | PLoS ONE 8(2): e55181 (2013) | - |
dc.identifier.uri | http://hdl.handle.net/10261/122793 | - |
dc.description.abstract | In mammalian visceral organs, vascular smooth muscle cells (VSMCs) originate from an epithelial-to-mesenchymal transition (EMT) of embryonic mesothelial cells (MCs). The ability of adult MCs to recapitulate EMT and to acquire smooth muscle (SM) markers upon provasculogenic culture suggested they might retain embryonic vasculogenic differentiation potential. However, it remains unknown whether adult MCs-derived SM-like cells may acquire specific vascular SM lineage markers and the functionality of differentiated contractile VSMCs. Here, we describe how a gentle trypsinization of adult mouse uterine cords could selectively detach their outermost uterine mesothelial layer cells. As other MCs; uterine MCs (UtMCs) uniformly expressed the epithelial markers β-catenin, ZO-1, E-cadherin, CD54, CD29, and CK18. When cultured in a modified SM differentiation media (SMDM) UtMCs initiated a loss of epithelial characteristics and gained markers expression of EMT (Twist, Snail, and Slug), stem and progenitor (Nanog, Sox2, C-kit, Gata-4, Isl-1, and nestin), SM (α-SMA, calponin, caldesmon, SM22α, desmin, SM-MHC, and smoothelin-B) and cardiac (BMP2, BMP4, ACTC1, sACTN, cTnI, cTnT, ANF, Cx43, and MLC2a). UtMCs repeatedly subcultured in SMDM acquired differentiated VSM-like characteristics and expressed smoothelin-B in the typical stress-fiber pattern expression of contractile VSMCs. Relevantly, UtMCs-derived VSM-like cells could generate >mechanical force> to compact collagen lattices and displayed in diverse degree voltage (K+) and receptor (endothelin-1, oxytocin, norepinephrine, carbachol and vasopressin)-induced [Ca2+]i rises and contraction. Thus, we show for the first time that UtMCs could recapitulate in vitro differentiative events of early cardiovascular differentiation and transdifferentiate in cells exhibiting molecular and functional characteristics of VSMCs. © 2013 Lachaud et al. | - |
dc.description.sponsorship | Authors are supported by the Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía (Grant PI-0022/2008); Consejería de Innovación Ciencia y Empresa, Junta de Andalucía (Grant CTS-6505; INP-2011-1615-900000); FEDER co-funded grants from Instituto de Salud Carlos III (Red TerCel-Grant RD06/0010/0025; PI10/00964) and the Ministry of Health and Consumer Affairs (Advanced Therapies Program Grant TRA-120). CIBERDEM is an initiative of the Instituto de Salud Carlos III. | - |
dc.publisher | Public Library of Science | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Functional Vascular Smooth Muscle-like Cells Derived from Adult Mouse Uterine Mesothelial Cells | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1371/journal.pone.0055181 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0055181 | - |
dc.date.updated | 2015-09-29T12:55:27Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Fundación Progreso y Salud | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Instituto de Salud Carlos III | - |
dc.contributor.funder | Ministerio de Sanidad y Consumo (España) | - |
dc.contributor.funder | Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.identifier.pmid | 23405120 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
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Functional_Vascular_Smooth_Lachaud.pdf | 7,05 MB | Adobe PDF | Visualizar/Abrir |
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