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dc.contributor.authorMarín Velázquez, Juan Antonioes_ES
dc.contributor.authorGarcía Martín, Elenaes_ES
dc.contributor.authorLorente Alonso, Pilares_ES
dc.contributor.authorAndreu Puyal, Pilares_ES
dc.contributor.authorArbeloa Matute, Aranchaes_ES
dc.identifier.citationMarín JA, García E, Lorente P, Andreu P, Arbeloa A. A novel approach for propagation of recalcitrant pistachio cultivars that sidesteps rooting by ex vitro grafting of tissue cultured shoot tips (Article in Press). Plant Cell, Tissue and Organ Culture 124 (1): 191-200 (2016)es_ES
dc.description10 Pags.- 5 Figs.- 1 Tabl. The definitive version is available at: http://link.springer.com/journal/11240es_ES
dc.description.abstractPistachio (Pistacia vera L.) cultivars need to be grafted on suitable rootstocks for their propagation, but grafting success is uncertain, preventing producers to meet plant demand. Thus, there is a need for a better grafting method that in addition to increase success rate, allows us an early detection of success. Here, we have developed an alternative grafting technique that achieves these goals using young potted seedlings of terebinth rootstock and reduced size micropropagated scions from commercial cultivars. During micropropagation of pistachio cultivars we studied the effects of the application of antioxidants together with the growth regulators meta-topolin and paclobutrazol on in vitro cultures to overcome the issues identified in the different phases of micropropagation: culture initiation, multiplication and rooting. Grafting succeeded using scions derived from in vitro cultured micro-shoots both from acclimated and non-acclimated plants. With this protocol, up to 75.7 % successful grafts were obtained, and grafted plants resumed growth from day 3, being ready for transplant a few months later. Sustained on our results, we present a simple and reliable system to reduce production time by ex-vitro grafting of shoot-tips from in vitro cultures onto 4 weeks old terebinth (Pistacia terebinthus L.) rootstock seedlings grown in pots.es_ES
dc.description.sponsorshipThis work was partly funded by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria-FEDER RTA2014-00056-C02 Project and by Group A-43 grant by the Government of Aragon.es_ES
dc.subjectPistacia veraes_ES
dc.subjectClonal propagationes_ES
dc.subjectIn vitro culturees_ES
dc.subjectShoot tip necrosises_ES
dc.titleA novel approach for propagation of recalcitrant pistachio cultivars that sidesteps rooting by ex vitro grafting of tissue cultured shoot tipses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)es_ES
dc.contributor.funderGobierno de Aragónes_ES
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