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Title

Desiccated doubled-haploid embryos obtained from microspore culture of barley cv. Igri

AuthorsRyan, A. B.; Castillo Alonso, Ana María ; Vallés Brau, María Pilar ; Sanz Madoz, José Miguel ; Cistué Sola, Luis
KeywordsBarley
Desiccation
Embryos
Doubled haploids
Microspores
Issue DateAug-1999
PublisherSpringer
CitationPlant Cell Reports 18 (11): 924-928 (1999)
AbstractBarley microspore-derived doubled-haploid embryos have been produced in vitro. The development of embryo desiccation technology will allow long-term storage, germplasm preservation and low delivery cost. Treatment of the microspore-derived embryos was essential to induce desiccation tolerance and to arrest further development and plant regeneration. At the concentrations used, a treatment with trehalose was more efficient than with sucrose, and mannitol was harmful to the embryos. Up to 80% of the desiccated embryos produced complete green plants when transferred to regeneration medium, by the application of a 0.6 m trehalose or a 10–5 m abscisic acid treatment to the embryos in the culture induction medium. The morphology of these plants was similar to plants produced directly from non-desiccated embryos.
Description5 Pags.- 4 Tabls.- 4 Figs.
Publisher version (URL)http://dx.doi.org/10.1007/s002990050685
URIhttp://hdl.handle.net/10261/122119
DOI10.1007/s002990050685
ISSN0721-7714
E-ISSN1432-203X
Appears in Collections:(EEAD) Artículos
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