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Title

A genome-wide function of THSC/TREX-2 at active genes prevents transcription–replication collisions

AuthorsSantos-Pereira, José M. ; García-Rubio, María L. ; González-Aguilera, Cristina ; Luna, Rosa ; Aguilera, Andrés
Issue Date7-Oct-2014
PublisherOxford University Press
CitationNucleic Acids Research 42(19): 12000-12014 (2014)
AbstractThe THSC/TREX-2 complex of Saccharomyces cerevisiae mediates the anchoring of transcribed genes to the nuclear pore, linking transcription elongation with mRNA export and genome stability, as shown for specific reporters. However, it is still unknown whether the function of TREX-2 is global and the reason for its relevant role in genome integrity. Here, by studying two TREX-2 representative subunits, Thp1 and Sac3, we show that TREX-2 has a genome-wide role in gene expression. Both proteins show similar distributions along the genome, with a gradient disposition at active genes that increases towards the 3 end. Thp1 and Sac3 have a relevant impact on the expression of long, G+C-rich and highly transcribed genes. Interestingly, replication impairment detected by the genome-wide accumulation of the replicative Rrm3 helicase is increased preferentially at highly expressed genes in the thp1Δ and sac3Δ mutants analyzed. Therefore, our work provides evidence of a function of TREX-2 at the genome-wide level and suggests a role for TREX-2 in preventing transcription– replication conflicts, as a source of genome instability derived from a defective messenger ribonucleoprotein particle (mRNP) biogenesis.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gku906
URIhttp://hdl.handle.net/10261/121620
DOI10.1093/nar/gku906
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(CABIMER) Artículos
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