CAPILLARY ELECTROPHORESIS USING COPOLYMERS OF DIFFERENT COMPOSITION AS PHYSICAL COATINGS. A COMPARATIVE STUDY.

Abstract

In this work, a comparative study on the use of different polymers as physically adsorbed coatings for Capillary Electrophoresis (CE) is presented. It is demonstrated that the use of ad hoc synthesized polymers as coatings allows tailoring the electroosmotic flow (EOF) in CE increasing the flexibility of this analytical technique. Namely, different polymers were synthesized at our laboratory using different percentages of ethylpyrrolidine methacrylate (EpyM) and N,N-dimethylacrylamide (DMA). Thus, by modifying the percentage of EpyM and DMA monomers it is possible to manipulate the positive charge of the copolymer, varying the global electrical charge on the capillary wall and with that the EOF. These coated capillaries are obtained by simply flushing a given EpyM-DMA aqueous solution into bare silica capillaries. It is shown that by using these coated capillaries at adequate pHs, faster or more resolved CE separations can be achieved depending on the requirements of each analysis. Moreover, it is demonstrated that these coated capillaries reduce the electrostatic adsorption of basic proteins onto the capillary wall. Furthermore, EpyM-DMA coatings allow the reproducible chiral separation of enantiomers through the partial filling technique. The EpyM-DMA coated capillaries are demonstrated to provide reproducible EOF values independently of the pH and polymer composition with % RSD values lower than 2 % for the same day. It is also demonstrated that the coating procedure is reproducible between capillaries. The compatibility of this coating protocol with CE in microchips is discussed.