English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/12157
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

CAPILLARY ELECTROPHORESIS USING COPOLYMERS OF DIFFERENT COMPOSITION AS PHYSICAL COATINGS. A COMPARATIVE STUDY.

AuthorsGuillaume, L. Erny; Elvira, Carlos ; San Román, Julio ; Cifuentes, Alejandro
KeywordsCapillary coating
physically adsorbed
protein separation
chiral separation
Issue DateFeb-2006
PublisherWiley-VCH
CitationElectrophoresis, vol. 27, no5-6, pp. 1041-1049
AbstractIn this work, a comparative study on the use of different polymers as physically adsorbed coatings for Capillary Electrophoresis (CE) is presented. It is demonstrated that the use of ad hoc synthesized polymers as coatings allows tailoring the electroosmotic flow (EOF) in CE increasing the flexibility of this analytical technique. Namely, different polymers were synthesized at our laboratory using different percentages of ethylpyrrolidine methacrylate (EpyM) and N,N-dimethylacrylamide (DMA). Thus, by modifying the percentage of EpyM and DMA monomers it is possible to manipulate the positive charge of the copolymer, varying the global electrical charge on the capillary wall and with that the EOF. These coated capillaries are obtained by simply flushing a given EpyM-DMA aqueous solution into bare silica capillaries. It is shown that by using these coated capillaries at adequate pHs, faster or more resolved CE separations can be achieved depending on the requirements of each analysis. Moreover, it is demonstrated that these coated capillaries reduce the electrostatic adsorption of basic proteins onto the capillary wall. Furthermore, EpyM-DMA coatings allow the reproducible chiral separation of enantiomers through the partial filling technique. The EpyM-DMA coated capillaries are demonstrated to provide reproducible EOF values independently of the pH and polymer composition with % RSD values lower than 2 % for the same day. It is also demonstrated that the coating procedure is reproducible between capillaries. The compatibility of this coating protocol with CE in microchips is discussed.
Publisher version (URL)http://dx.doi.org/10.1002/elps.200500692
URIhttp://hdl.handle.net/10261/12157
DOI10.1002/elps.200500692
ISSN0173-0835
Appears in Collections:(IFI) Artículos
Files in This Item:
File Description SizeFormat 
Electrophoresis 27 _2006_ 1041-1049.pdf277,65 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.