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Proteotyping of human haptoglobin by MALDI-TOF profiling: Phenotype distribution in a population of toxic oil syndrome patients

AuthorsRodríguez Abreu, Carlos; Quero, Carmen CSIC ORCID ; Domínguez, Ana; Trigo, Miguel; Posada de la Paz, Manuel; Gelpí, Emili CSIC; Abián, Joaquín CSIC ORCID
KeywordsDisease markers
Haptoglobin polymorphism
MALDI-TOF proteotyping
Serum proteomics
Toxic oil syndrome
Issue Date18-Apr-2006
PublisherJohn Wiley & Sons
CitationProteomics 6(1): 272-281 (2006)
AbstractToxic oil syndrome (TOS) is a disease that appeared in Spain in 1981 as a consequence of the ingestion of an aniline-adulterated oil illegally marketed as edible. TOS affected more than 20 000 people and produced over 400 deaths in the first 18 months after the outbreak. There is evidence that genetic factors could play a role in the susceptibility of individuals towards the disease. Recently, we suggested that haptoglobin (Hp) polymorphism could also play a role in TOS. To provide a rapid method for high-throughput Hp phenotyping, we developed a two-step MALDI-TOF procedure that allows specific identification of the three common Hp chains. Resolution of the homologous α-1s and α-1f chains, which have a mass difference of only 0.043 Da, is obtained after guanidination of the protein with O-methylisourea. We applied this procedure to the study of the distribution of the Hp alleles HP(1s), HP(1f), HP2 in a control versus a TOS-affected population, both originally exposed to the toxic oil. The MALDI-TOF proteotyping method was validated by a parallel analysis of the serum samples by 2-DE. Data obtained from 54 TOS cases and 48 control individuals indicate significant differences in the distribution of Hp phenotypes in the two populations.
Description10 pages, 6 figures, 3 tables.-- PMID: 16544284 [PubMed].
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