Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/121456
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Purification and characterization of a pregastric esterase from a hygienized kid rennet paste |
Autor: | Calvo, Maria V. CSIC ORCID ; Fontecha, F. Javier CSIC ORCID ; Fontecha, F. Javier CSIC ORCID | Palabras clave: | Rennet paste Pregastric esterase Artisanal cheese |
Fecha de publicación: | 2004 | Editor: | American Dairy Science Association | Citación: | Journal of Dairy Science 87: 1132- 1142 (2004) | Resumen: | Rennet pastes obtained by maceration of gastric tissues from suckling kids are used traditionally to produce some artisanal cheeses in Spain. Besides milk-clotting function, rennet pastes provide proteolytic activity and lipolytic system, essentially pregastric, necessary in the development of piquant flavor typical of these cheeses. A simple and reproducible procedure allows us to obtain a standardized rennet paste that posses the desired activity and is of good microbiological quality. Concomitantly, a kid pregastric esterase (KPGE) was purified to homogeneity. The purification procedure was based on an aqueous extract of hygienized rennet paste (HRP), which was chromatographed on DEAE-Sepharose Fast Flow then adsorbed on phenyl superose followed by a re-chromatography on the same column. The final enzymatic preparation, where the overall activity recovery was 3%, showed a molecular mass of 53 kDa. The highest activity was determined on p-nitrophenyl butyrate, but marked hydrolysis was also detected on β-naphthyl caprylate. In contrast, low activity on tributyrin (substrate under emulsion form) was detected, thus confirming the esterase character of purified enzyme. | URI: | http://hdl.handle.net/10261/121456 | DOI: | 10.3168/jds.S0022-0302(04)73261-0 | Identificadores: | doi: 10.3168/jds.S0022-0302(04)73261-0 issn: 0022-0302 |
Aparece en las colecciones: | (IF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
15
checked on 25-mar-2024
WEB OF SCIENCETM
Citations
13
checked on 29-feb-2024
Page view(s)
321
checked on 18-abr-2024
Download(s)
118
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.