Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/121304
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Influence of anther pretreatment and culture medium composition on the production of barley doubled haploids from model and low responding cultivars |
Autor: | Cistué Sola, Luis CSIC ORCID ; Ramos López, Amelia; Castillo Alonso, Ana María CSIC ORCID | Fecha de publicación: | 1998 | Editor: | Kluwer Academic Publishers | Citación: | Plant Cell, Tissue and Organ Culture 55: 159-166 (1998) | Resumen: | Different pretreatments were given to anthers of barley before culturing, and their effects assessed on the frequency of embryos and green doubled haploid plants produced. Mannitol pretreatment was better than cold pretreatment for some low responding cultivars. Optimal concentration of mannitol for pretreatment depended on cultivar. Low responding genotypes needed a higher concentration of mannitol than responsive ones. The addition of Ficoll to liquid medium increased the number of embryos and green plants. The influence of the growth regulators 2,4-D and TIBA was assayed using ten cultivars of barley grown in Spain. The anti-auxin TIBA gave good embryo production with some of the low responding cultivars. Two row-type cultivars always produced higher number of embryos and green plantlets than six row-type. The application of these modifications to 10 F1 hybrids with potential agronomic value, allowed the production of almost 1000 doubled haploid plants from only 3500 anthers. Up to two doubled haploid plants per flower were produced from the cross Monlon x Sonja. | URI: | http://hdl.handle.net/10261/121304 | DOI: | 10.1023/A:1006130028396 | Identificadores: | doi: 10.1023/A:1006130028396 issn: 0167-6857 |
Aparece en las colecciones: | (EEAD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
45
checked on 25-mar-2024
WEB OF SCIENCETM
Citations
38
checked on 26-feb-2024
Page view(s)
254
checked on 21-abr-2024
Download(s)
94
checked on 21-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.