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dc.contributor.authorBelenguer, Álvaro-
dc.contributor.authorBalcells, Joaquín-
dc.contributor.authorGuada, Jose A.-
dc.contributor.authorDecoux, Marc-
dc.contributor.authorMilne, Eric-
dc.identifier.citationBritish Journal of Nutrition 94(5): 763-770 (2005)en_US
dc.description8 pages, 6 tables, 2 figures.-- PMID: 16277780 [PubMed]en_US
dc.description.abstractTo study the absorption of microbial lysine in growing rabbits, a labelled diet (supplemented with (15)NH4Cl) was administered to six animals (group ISOT); a control group (CTRL, four rabbits) received a similar, but unlabelled, diet. Diets were administered for 30 d. An additional group of six animals were fed the unlabelled diet for 20 d and then the labelled diet for 10 d while wearing a neck collar to avoid caecotrophy (group COLL), in order to discriminate it from direct intestinal absorption. At day 30 animals were slaughtered and caecal bacteria and liver samples taken. The (15)N enrichment in amino acids of caecal bacteria and liver were determined by GC-combustion/isotope ratio MS. Lysine showed a higher enrichment in caecal microflora (0.925 atom% excess, APE) than liver (0.215 APE) in group ISOT animals, confirming the double origin of body lysine: microbial and dietary. The COLL group showed a much lower enrichment in tissue lysine (0.007 (se 0.0029) APE for liver). Any enrichment in the latter animals was due to direct absorption of microbial lysine along the digestive tract, since recycling of microbial protein (caecotrophy) was avoided. In such conditions liver enrichment was low, indicating a small direct intestinal absorption. From the ratio of [(15)N]lysine enrichment between liver and bacteria the contribution of microbes to body lysine was estimated at 23 %, with 97 % of this arising through caecotrophy. Absorption of microbial lysine through caecotrophy was 119 (se 4.0) mg/d, compared with 406 (se 1.8) mg/d available from the diet. This study confirms the importance of caecotrophy in rabbit nutrition (15 % of total protein intake).en_US
dc.description.sponsorshipThe work was financed by Agribrands Europe España SA, Barcelona, Spain and the Spanish Ministry of Science through Projects PTR 95/0405-OP and PB98-01 601. A. B. was supported by a fellowship from the Spanish Science and Technology Ministry.en_US
dc.format.extent199204 bytes-
dc.publisherCambridge University Pressen_US
dc.subjectMicrobial lysineen_US
dc.titleProtein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolismen_US
dc.description.peerreviewedPeer revieweden_US
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