English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/118155
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Determination of cholesterol oxides content in milk products by solid phase extraction and gas chromatography-mass spectromety

AuthorsCalvo, Maria V. ; Ramos, Lourdes ; Fontecha, F. Javier
KeywordsSPE
GC-MS
Milk products
Cholesterol oxidation products (COPs)
Issue Date2003
PublisherJohn Wiley & Sons
CitationJournal of Separation Science 26: 927- 931 (2003)
AbstractA method for quick and reliable analysis of cholesterol oxidation products (COPs) in dairy products has been developed. After lipid extraction, fat was transesterified under mild conditions to avoid degradation of the target compounds. Isolation of the COPs studied from other components in the lipid fraction was carried out on an aminopropyl SPE cartridge. Finally, analytes were analysed by GC-MS without derivatisation. The method developed provides high specificity and good sensitivity, allowing the direct and unambiguous determination of the underivatized COPs investigated. Application of the method to dairy food analysis revealed the presence of COPs in powder milks and milk-based infant formulas commercially available in Spain, showing that attention should be focused on reduction of cholesterol oxidation levels during both the processing and the storage of these types of foodstuffs.
URIhttp://hdl.handle.net/10261/118155
DOI10.1002/jssc.200301249
Identifiersdoi: 10.1002/jssc.200301249
issn: 1615-9306
Appears in Collections:(IF) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.