English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/117970
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Crystal structure of Hcp from Acinetobacter baumannii: a component of the type VI secretion system

AuthorsRuiz, Federico M. ; Santillana, Elena ; Spínola-Amilibia, Mercedes ; Torreira, Eva ; Culebras, Esther; Romero, Antonio
Issue Date16-Jun-2015
PublisherPublic Library of Science
CitationPLoS ONE | DOI:10.1371/journal.pone.0129691 June 16, 2015
AbstractThe type VI secretion system (T6SS) is a bacterial macromolecular machine widely distributed in Gram-negative bacteria, which transports effector proteins into eukaryotic host cells or other bacteria. Membrane complexes and a central tubular structure, which resembles the tail of contractile bacteriophages, compose the T6SS. One of the proteins forming this tube is the hemolysin co-regulated protein (Hcp), which acts as virulence factor, as transporter of effectors and as a chaperone. In this study, we present the structure of Hcp from Acinetobacter baumannii, together with functional and oligomerization studies. The structure of this protein exhibits a tight β barrel formed by two β sheets and flanked at one side by a short α-helix. Six Hcp molecules associate to form a donut-shaped hexamer, as observed in both the crystal structure and solution. These results emphasize the importance of this oligomerization state in this family of proteins, despite the low similarity of sequence among them. The structure presented in this study is the first one for a protein forming part of a functional T6SS from A. baumannii. These results will help us to understand the mechanism and function of this secretion system in this opportunistic nosocomial pathogen.
Description16 p.-6 fig.-1 tab.
Publisher version (URL)http://dx.doi.org/ 10.1371/journal.pone.0129691
URIhttp://hdl.handle.net/10261/117970
DOI10.1371/journal.pone.0129691
ISSN1932-6203
E-ISSN1932-6203
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
PLOS_ONE_2015_Ruiz,Federico M..pdf5,21 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.