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Closed Access item Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor

Authors:Mauriz, Elba
Calle Martín, Ana
Abad, Antonio
Montoya, Ángel
Hildebrandt, Alain
Barceló, Damià
Lechuga, Laura M.
Keywords:Surface plasmon resonance (SPR), Immunosensor, Self-assembled monolayer, Carbaryl, Validation, Environmental analysis
Issue Date:15-May-2006
Citation:Biosensors & Bioelectronics 21(11): 2129-2336 (2006)
Abstract:The analysis of carbaryl in natural water samples was accomplished using a portable immunosensor based on surface plasmon resonance (SPR) technology. The assay was based on a binding inhibition immunoassay format with the analyte derivative covalently immobilized on the sensor surface. An alkanethiol self-assembled monolayer (SAM) was formed onto the gold-coated sensor surface to allow the reusability of the same sensing surface during 220 regeneration cycles. Reproducibility was evaluated by performing three independent assays in triplicate on 3 different days. The batch-assay variability was also calculated using three different gold-coated sensor surfaces. The intra- and inter-day relative standard deviation were 8.6 and 15.3%, respectively, whilst a variation of 7.4% in assay sensitivity was obtained by employing different sensor chips. The lowest detection limit, calculated as the concentration providing a 10% decrease of the blank signal, was of 1.38 μg L−1. Matrix effects were also evaluated in different water types, showing I50 values (carbaryl concentrations that produced a 50% decrease of the blank signal) within the range of carbaryl standard curves in distilled water (2.78–3.55 μg L−1). The carbaryl immunoassay performance was validated with respect to conventional high-performance liquid chromatography–mass spectrometry (HPLC–MS). The correlation between methods was in good agreement (r2 = 0.998, 0.999 and 0.999) for the three types of natural water samples tested. A complete assay cycle, including regeneration, is accomplished in 20 min. All measurements were carried out with the SPR sensor system (β-SPR) commercialised by the company SENSIA, SL (Spain). The small size and low-time of response of the β-SPR platform would allow its utilization in real contaminated locations.
Description:8 pages, 3 tables, 5 figures.-- PMID: 16309901 [PubMed].-- Available online Nov 23, 2005.
Publisher version (URL):http://dx.doi.org/10.1016/j.bios.2005.10.013
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