English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/117106
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Amino acid transporters and release of hydrophobic amino acids in the heterocyst-forming cyanobacterium anabaena sp. strain PCC 7120

AuthorsPernil, Rafael ; Picossi, Silvia ; Herrero, Antonia ; Flores, Enrique ; Mariscal, Vicente
KeywordsABC-type transporters
Amino acid diffusion
Cyanobacteria
Membrane transport
Issue Date2015
PublisherMultidisciplinary Digital Publishing Institute
CitationLife 5(2): 1282-1300 (2015)
AbstractAnabaena sp. strain PCC 7120 is a filamentous cyanobacterium that can use inorganic compounds such as nitrate or ammonium as nitrogen sources. In the absence of combined nitrogen, it can fix N2 in differentiated cells called heterocysts. Anabaena also shows substantial activities of amino acid uptake, and three ABC-type transporters for amino acids have been previously characterized. Seven new loci encoding predicted amino acid transporters were identified in the Anabaena genomic sequence and inactivated. Two of them were involved in amino acid uptake. Locus alr2535-alr2541 encodes the elements of a hydrophobic amino acid ABC-type transporter that is mainly involved in the uptake of glycine. ORF all0342 encodes a putative transporter from the dicarboxylate/amino acid:cation symporter (DAACS) family whose inactivation resulted in an increased uptake of a broad range of amino acids. An assay to study amino acid release from Anabaena filaments to the external medium was set up. Net release of the alanine analogue α-aminoisobutyric acid (AIB) was observed when transport system N-I (a hydrophobic amino acid ABC-type transporter) was engaged in the uptake of a specific substrate. The rate of AIB release was directly proportional to the intracellular AIB concentration, suggesting leakage from the cells by diffusion
Publisher version (URL)http://dx.doi.org/10.3390/life5021282
URIhttp://hdl.handle.net/10261/117106
DOI10.3390/life5021282
E-ISSN2075-1729
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
life-05-01282-v2 (1).pdf1,23 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.