Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/117011
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Comparison of manual and semi-automatic DNA extraction protocols for the barcoding characterization of hematophagous louse flies (Diptera: Hippoboscidae) |
Autor: | Gutiérrez-López, Rafael; Martínez de la Puente, Josué CSIC ORCID; Gangoso, Laura CSIC ORCID; Soriguer, Ramón C. CSIC ORCID CVN ; Figuerola, Jordi CSIC ORCID | Palabras clave: | Parasites Ornithophila gestroi Hippoboscids COI Barcoding of life DNA extraction methods |
Fecha de publicación: | 2015 | Editor: | Wiley-Blackwell | Citación: | Journal of Vector Ecology 40: 11- 15 (2015) | Resumen: | © 2015 The Society for Vector Ecology. The barcoding of life initiative provides a universal molecular tool to distinguish animal species based on the amplification and sequencing of a fragment of the subunit 1 of the cytochrome oxidase (COI) gene. Obtaining good quality DNA for barcoding purposes is a limiting factor, especially in studies conducted on small-sized samples or those requiring the maintenance of the organism as a voucher. In this study, we compared the number of positive amplifications and the quality of the sequences obtained using DNA extraction methods that also differ in their economic costs and time requirements and we applied them for the genetic characterization of louse flies. Four DNA extraction methods were studied: chloroform/isoamyl alcohol, HotShot procedure, Qiagen DNeasy® Tissue and Blood Kit and DNA Kit Maxwell® 16LEV. All the louse flies were morphologically identified as Ornithophila gestroi and a single COI-based haplotype was identified. The number of positive amplifications did not differ significantly among DNA extraction procedures. However, the quality of the sequences was significantly lower for the case of the chloroform/isoamyl alcohol procedure with respect to the rest of methods tested here. These results may be useful for the genetic characterization of louse flies, leaving most of the remaining insect as a voucher. | URI: | http://hdl.handle.net/10261/117011 | DOI: | 10.1111/jvec.12127 | Identificadores: | doi: 10.1111/jvec.12127 issn: 1948-7134 |
Aparece en las colecciones: | (EBD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Comparison of manual and semiautomatic DNA extraction protocols for the barcoding characterization of hematophagous louse flies (Diptera. Hippobosc.pdf | 233,6 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
28
checked on 18-abr-2024
WEB OF SCIENCETM
Citations
27
checked on 28-feb-2024
Page view(s)
551
checked on 23-abr-2024
Download(s)
896
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.