Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/117009COMPARTIR / EXPORTAR:
 SHARE
 CORE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Tungstate-targeting of BKαβ1 channels tunes ERK phosphorylation and cell proliferation in human vascular smooth muscle |
Autor: | Fernández-Mariño, Ana Isabel; Cidad, Pilar CSIC ORCID; López-López, José R. CSIC ORCID; Pérez-García, M. Teresa CSIC ORCID; Fernández-Fernández, José M. CSIC | Fecha de publicación: | 2015 | Editor: | Public Library of Science | Citación: | PLoS ONE 10(2): 30118148 (2015) | Resumen: | Despite the substantial knowledge on the antidiabetic, antiobesity and antihypertensive actions of tungstate, information on its primary target/s is scarce. Tungstate activates both the ERK1/2 pathway and the vascular voltage- and Ca2+-dependent large-conductance BKαβ smooth muscle cell (VSMC) proliferation and function, respectively. Here, we have assessed the possible involvement of BKαβ1 channels in the tungstate-induced ERK phosphorylation and its relevance for VSMC proliferation. Western blot analysis in HEK cell lines showed that expression of vascular BKαβ1 channels potentiates the tungstate-induced ERK1/2 phosphorylation in a Gi/o proteindependent manner. Tungstate activated BKαβ1 channels upstream of G proteins as channel activation was not altered by the inhibition of G proteins with GDPβ S or pertussis toxin. Moreover, analysis of Gi/o protein activation measuring the FRET among heterologously expressed G i protein subunits suggested that tungstate-targeting of BK αβ1 channels promotes G protein activation. Single channel recordings on VSMCs from wild-type and β1-knockout mice indicated that the presence of the regulatory β1 subunit was essential for the tungstate-mediated activation of BK channels in VSMCs. Moreover, the specific BK channel blocker iberiotoxin lowered tungstate-induced ERK phosphorylation by 55% and partially reverted (by 51%) the tungstate-produced reduction of platelet-derived growth factor (PDGF)-induced proliferation in human VSMCs. Our observations indicate that tungstate-targeting of BK αβ1 channels promotes activation of PTX-sensitive Gi proteins to enhance the tungstate-induced phosphorylation of ERK, and inhibits PDGF-stimulated cell proliferation in human vascular smooth muscle. | Descripción: | This is an open access article distributed under the terms of the Creative Commons Attribution License.-- et al. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0118148 | URI: | http://hdl.handle.net/10261/117009 | DOI: | 10.1371/journal.pone.0118148 | Identificadores: | doi: 10.1371/journal.pone.0118148 issn: 1932-6203 |
| Aparece en las colecciones: | (IBGM) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Tungstate-Targeting.pdf | 1,09 MB | Adobe PDF |  Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
4
checked on 04-mar-2024
SCOPUSTM
Citations
12
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
9
checked on 28-feb-2024
Page view(s)
273
checked on 19-abr-2024
Download(s)
276
checked on 19-abr-2024
