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Title

Altered human gut dendritic cell properties in ulcerative colitis are reversed by Lactobacillus plantarum extracellular encrypted peptide STp

AuthorsAl-Hassi, Hafid O.; Mann, Elizabeth R.; Fernández-Salazar, Luis; Garrote, José Antonio ; Arranz, Eduardo ; Margolles Barros, Abelardo ; Knight, Stella C.; Bernardo, David
KeywordsMicrobiota
Postbiotics
STp
Ulcerative colitis
Dendritic cells
Issue Date2014
PublisherJohn Wiley & Sons
CitationMolecular Nutrition and Food Research 58(5): 1132-1143 (2014)
Abstract[Scope]: The human/microbiota cross-talk is partially mediated by bacteria-derived peptides like Serine-Threonine peptide (STp), which is resistant to gut proteolysis, is found in the human healthy colon and induces regulatory properties on gut dendritic cells (DCs); here we characterized human gut DC in ulcerative colitis (UC) patients and studied the effect of STp on their properties. [Methods and results]: Human colonic DC from healthy controls and UC patients were isolated, conditioned for 24 h +/- STp and characterized by flow cytometry, immunohistochemistry, and electron microscopy. Expression of immature DC markers DC-SIGN and ILT3, and Toll-like receptors were increased on gut UC-DC. Langerin (involved in phagocytosis), lymph node homing marker CCR7, and activation markers CD40/CD80/CD86 were decreased in UC. Gut DC had restricted stimulatory capacity for T-cells in UC. Conditioning of DC with STp in vitro reduced Toll-like receptor expression, increased CD40 and CD80 expression, and restored their stimulatory capacity. [Conclusion]: Colonic DCs display an abnormal immature phenotype in UC, which was partially restored following STp treatment. Bacteria-derived metabolites, like STp, seem to have a role in gut homeostasis that is missing in UC so they might lead a new era of probiotic products setting the basis for nondrug dietary therapy in inflammatory bowel disease. © 2013 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA Weinheim.
DescriptionThis is an open access article under the terms of the Creative Commons Attribution License.-- et al.
Publisher version (URL)http://dx.doi.org/10.1002/mnfr.201300596
URIhttp://hdl.handle.net/10261/116904
DOI10.1002/mnfr.201300596
Identifiersdoi: 10.1002/mnfr.201300596
issn: 1613-4125
e-issn: 1613-4133
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