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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/116863
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dc.contributor.authorHidalgo, Pedro I.-
dc.contributor.authorMontero, Olimpio-
dc.contributor.authorMartín, Juan-Francisco-
dc.date.accessioned2015-06-19T09:07:14Z-
dc.date.available2015-06-19T09:07:14Z-
dc.date.issued2014-
dc.identifierdoi: 10.1016/j.fgb.2013.10.009-
dc.identifierissn: 1087-1845-
dc.identifiere-issn: 1096-0937-
dc.identifier.citationFungal Genetics and Biology 62: 11-24 (2014)-
dc.identifier.urihttp://hdl.handle.net/10261/116863-
dc.descriptionet al.-
dc.description.abstractThe PR-toxin is a potent mycotoxin produced by Penicillium roqueforti in moulded grains and grass silages and may contaminate blue-veined cheese. The PR-toxin derives from the 15 carbon atoms sesquiterpene aristolochene formed by the aristolochene synthase (encoded by ari1). We have cloned and sequenced a four gene cluster that includes the ari1 gene from P. roqueforti. Gene silencing of each of the four genes (named prx1 to prx4) resulted in a reduction of 65-75% in the production of PR-toxin indicating that the four genes encode enzymes involved in PR-toxin biosynthesis. Interestingly the four silenced mutants overproduce large amounts of mycophenolic acid, an antitumor compound formed by an unrelated pathway suggesting a cross-talk of PR-toxin and mycophenolic acid production. An eleven gene cluster that includes the above mentioned four prx genes and a 14-TMS drug/H+ antiporter was found in the genome of Penicillium chrysogenum. This eleven gene cluster has been reported to be very poorly expressed in a transcriptomic study of P. chrysogenum genes under conditions of penicillin production (strongly aerated cultures). We found that this apparently silent gene cluster is able to produce PR-toxin in P. chrysogenum under static culture conditions on hydrated rice medium. Noteworthily, the production of PR-toxin was 2.6-fold higher in P. chrysogenum npe10, a strain deleted in the 56.8kb amplifiable region containing the pen gene cluster, than in the parental strain Wisconsin 54-1255 providing another example of cross-talk between secondary metabolite pathways in this fungus. A detailed PR-toxin biosynthesis pathway is proposed based on all available evidence. © 2013 Elsevier Inc.-
dc.description.sponsorshipThis work was supported by a Grant of the European Union (EUROFUNGBASE, LSSG-CT-2005-018964). Pedro Hidalgo received a fellowship from the AECID (Agencia Española de Cooperación Internacional para el Desarrollo).-
dc.publisherElsevier-
dc.rightsclosedAccess-
dc.subjectPenicillium-
dc.subjectCross-pathways regulation-
dc.subjectGene silencing-
dc.subjectSilent cluster-
dc.subjectPR-toxin-
dc.subjectMycotoxins-
dc.titleMolecular characterization of the PR-toxin gene cluster in Penicillium roqueforti and Penicillium chrysogenum: Cross talk of secondary metabolite pathways-
dc.typeartículo-
dc.identifier.doi10.1016/j.fgb.2013.10.009-
dc.date.updated2015-06-19T09:07:14Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Asuntos Exteriores y Cooperación (España)-
dc.relation.csicSí-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003767es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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