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Abrc3, a response regulator that activates the antibiotic production in Streptomyces coelicolor

AuthorsRico, Sergio ; Yepes, Ana ; Rodríguez, Héctor ; Santamaría, Ramón I. ; Díaz, Margarita
Antibiotic production
Two-component systems
Issue Date2014
Abstract[Introduction]: Two-component systems (TCSs) trigger important global pathways in the regulation of antibiotic production in Streptomyces coelicolor. The conserved AbrC1/C2/C3 system is an atypical TCS composed of two histidine kinases and one response regulator and it is involved in the activation of the antibiotic production and morphological differentiation. In this work, we have studied the role of the AbrC3 Response Regulator in the production of antibiotics. [Experimental and Results]: The mutant strain ΔabrC3 is unable to produce actinorhodin (ACT) in nutrient broth; moreover, transcriptomic analysis of this mutant and the parent strain has shown that the expression of most act cluster genes is significantly reduced in the ΔabrC3 strain. Chromatin immunoprecipitation with microarray technology (ChIP-chip) analysis and electrophoretic mobility shift assays (EMSAs) have revealed that AbrC3 directly controls ACT production by binding to the actII-ORF4 promoter region (SARP pathway-specific regulator of the act cluster), and this binding is dependent on the sequence 5’-GAAGGCGACC-3’. Overexpression of the abrC3 gen increased the ACT production in S. coelicolor and induced the production of this antibiotic in S. lividans, specie that does not produce ACT in the most common laboratory conditions. We also have tested the effect of the overexpression of abrC3 in other Streptomyces species, and for example, S. argillaceus produces new secondary metabolites not determined yet. For these reasons, genetic manipulation of AbrC3 can contribute to the rational design of new hyperproducer host strains and strategies involving TCSs could be used to unveil new antimicrobial molecules that are not produced under laboratory culture conditions.
DescriptionResumen del póster presentado al Congreso del ANQUE-ICCE-BIOTEC: Science, the key for a better life; celebrado en Madrid del 1 al 4 de julio de 2014.
Appears in Collections:(IBFG) Comunicaciones congresos
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