Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/116212
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1 |
Autor: | Curto, María Ángeles CSIC; Sharifmoghadam, M. R. CSIC ORCID; Calpena, Eduardo CSIC ORCID; León, Nagore de CSIC; Hoya, Marta CSIC ORCID; Doncel, Cristina CSIC ORCID; Leatherwood, Janet; Valdivieso, María Henar CSIC ORCID | Palabras clave: | Mating Membrane Prm1 Yeast Cell fusion |
Fecha de publicación: | 2014 | Editor: | Genetics Society of America | Citación: | Genetics 196(4): 1059-1076 (2014) | Resumen: | The involvement of Schizosaccharomyces pombe prm1+ in cell fusion during mating and its relationship with other genes required for this process have been addressed. S. pombe prm1Δ mutant exhibits an almost complete blockade in cell fusion and an abnormal distribution of the plasma membrane and cell wall in the area of cell-cell interaction. The distribution of cellular envelopes is similar to that described for mutants devoid of the Fig1-related claudin-like Dni proteins; however, prm1+ and the dni+ genes act in different subpathways. Time-lapse analyses show that in the wild-type S. pombe strain, the distribution of phosphatidylserine in the cytoplasmic leaflet of the plasma membrane undergoes some modification before an opening is observed in the cross wall at the cell- cell contact region. In the prm1Δ mutant, this membrane modification does not take place, and the cross wall between the mating partners is not extensively degraded; plasma membrane forms invaginations and fingers that sometimes collapse/retract and that are sometimes strengthened by the synthesis of cell-wall material. Neither prm1Δ nor prm1Δ dniΔ zygotes lyse after cell-cell contact in medium containing and lacking calcium. Response to drugs that inhibit lipid synthesis or interfere with lipids is different in wild-type, prm1Δ, and dni1Δ strains, suggesting that membrane structure/organization/dynamics is different in all these strains and that Prm1p and the Dni proteins exert some functions required to guarantee correct membrane organization that are critical for cell fusion. © 2014 by the Genetics Society of America. | URI: | http://hdl.handle.net/10261/116212 | DOI: | 10.1534/genetics.113.159558 | Identificadores: | doi: 10.1534/genetics.113.159558 issn: 0016-6731 e-issn: 1943-2631 |
Aparece en las colecciones: | (IBFG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
11
checked on 13-abr-2024
SCOPUSTM
Citations
19
checked on 17-abr-2024
WEB OF SCIENCETM
Citations
19
checked on 25-feb-2024
Page view(s)
276
checked on 18-abr-2024
Download(s)
75
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.