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Título: | Affinity chromatography of proteinases using bacitracin immobilized to porous glass beads |
Autor: | Fontecha, F. Javier CSIC ORCID ; Requena, Teresa CSIC ORCID ; Swaisgood, H. E | Fecha de publicación: | 1996 | Editor: | Blackwell Publishing | Citación: | Letters in Applied Microbiology 22: 371- 374 (1996) | Resumen: | This study describes an affinity chromatography procedure for proteinase purification using bioselective binding to immobilized bacitracin. By coupling bacitracin to controlled-pore glass (CPG) beads, an affinity matrix was obtained that permitted rapid purification of proteinases under conditions that minimize autolysis. Bacitracin-CPG was used to bioselectively adsorb the extracellular proteinase secreted by Enterococcus faecalis var. liquefaciens IFPL 383. The overall purification obtained with this procedure was 5149-fold. The ability of bacitracin-CPG to bind other proteinases was examined using various commercial proteinases. The specific activities of subtilin BPN' and proteinase K were increased by bioselective adsorption and excellent recoveries of all proteinases applied were obtained. | URI: | http://hdl.handle.net/10261/115826 | DOI: | 10.1111/j.1472-765X.1996.tb01181 | Identificadores: | doi: 10.1111/j.1472-765X.1996.tb01181 issn: 0266-8254 |
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