English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/115826
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Affinity chromatography of proteinases using bacitracin immobilized to porous glass beads

Autor Fontecha, F. Javier ; Requena, Teresa ; Swaisgood, H. E
Fecha de publicación 1996
EditorBlackwell Publishing
Citación Letters in Applied Microbiology 22: 371- 374 (1996)
ResumenThis study describes an affinity chromatography procedure for proteinase purification using bioselective binding to immobilized bacitracin. By coupling bacitracin to controlled-pore glass (CPG) beads, an affinity matrix was obtained that permitted rapid purification of proteinases under conditions that minimize autolysis. Bacitracin-CPG was used to bioselectively adsorb the extracellular proteinase secreted by Enterococcus faecalis var. liquefaciens IFPL 383. The overall purification obtained with this procedure was 5149-fold. The ability of bacitracin-CPG to bind other proteinases was examined using various commercial proteinases. The specific activities of subtilin BPN' and proteinase K were increased by bioselective adsorption and excellent recoveries of all proteinases applied were obtained.
URI http://hdl.handle.net/10261/115826
DOI10.1111/j.1472-765X.1996.tb01181
Identificadoresdoi: 10.1111/j.1472-765X.1996.tb01181
issn: 0266-8254
Aparece en las colecciones: (IF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.