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Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance

AuthorsBernal, José ; Herrero, Miguel ; Velasco, Diego ; Elvira, Carlos ; Cifuentes, Alejandro
Issue Date2010
CitationISC 2010
AbstractIn capillary electrophoresis (CE), fused-silica capillary surface has long been known to cause adsorption problems during the analysis of high positively charged compounds (e.g., basic proteins) due to electrostatic interactions between the solute and the capillary wall. Moreover, the fused silica wall is also responsible of the irreproducibility frequently observed between injections and capillaries. The use of capillary coatings is a good strategy to avoid these harmful effects. In this work, the properties of four copolymers synthesized in our laboratory are studied as physically adsorbed coatings for capillary electrophoresis (CE). Namely, the four copolymers investigated were poly(N ethyl morpholine methacrylamide-co-N,N-dimethyl-acrylamide), poly(N ethyl pyrrolidine methacrylate-co-N,N-dimethylacrylamide), poly(N ethyl morpholine methacrylate-co-N,N-dimethylacrylamide) and poly(N ethyl pyrrolidine methacrylamide-co-N,N-dimethylacrylamide). The capillary coating is easily obtained by simply flushing into the tubing the dissolution containing the copolymer. The stability and reproducibility of each coating were tested for the same day, different days and different capillaries. It is demonstrated that the use of these coatings in CE can drastically reduce the analysis time and/or to improve the reproducibility of the separations. It is also shown that all the coated capillaries allow the separation of basic proteins by reducing their adsorption onto the capillary wall. Links between their molecular structure, physico-chemical properties and their performance as coatings in CE are discussed.
DescriptionResumen del póster presentado al 28th International Symposium on Chromatography celebrado en Valencia (España) del 12 al 16 de septiembre de 2010.
Appears in Collections:(CIAL) Comunicaciones congresos
(ICTP) Comunicaciones congresos
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