English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/113790
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Diversity and functional convergence of small noncoding RNAs in male germ cell differentiation and fertilization

AuthorsGarcía-López, Jesús; Alonso, Lola; Cárdenas, David B.; Artaza-Álvarez, Haydeé; Hourcade, Juan D.; Martínez, Sergio; Brieño-Enríquez, Miguel A.; Del Mazo, Jesús
KeywordssncRNAs
Germ cell development
miRNAs
piRNAs
snoRNAs
Next-generation sequencing
Issue Date24-Mar-2015
PublisherCold Spring Harbor Laboratory Press
CitationRNA (2015) 21: 946-962
AbstractThe small noncoding RNAs (sncRNAs) are considered as post-transcriptional key regulators of male germ cell development. In addition to microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs), other sncRNAs generated from small nucleolar RNAs (snoRNAs), tRNAs, or rRNAs processing may also play important regulatory roles in spermatogenesis. By next-generation sequencing (NGS), we characterized the sncRNA populations detected at three milestone stages in male germ differentiation: primordial germ cells (PGCs), pubertal spermatogonia cells, and mature spermatozoa. To assess their potential transmission through the spermatozoa during fertilization, the sncRNAs of mouse oocytes and zygotes were also analyzed. Both, microRNAs and snoRNA-derived small RNAs are abundantly expressed in PGCs but transiently replaced by piRNAs in spermatozoa and endo-siRNAs in oocytes and zygotes. Exhaustive analysis of miRNA sequence variants also shows an increment of noncanonical microRNA forms along male germ cell differentiation. RNAs-derived from tRNAs and rRNAs interacting with PIWI proteins are not generated by the ping-pong pathway and could be a source of primary piRNAs. Moreover, our results strongly suggest that the small RNAs-derived from tRNAs and rRNAs are interacting with PIWI proteins, and specifically with MILI. Finally,computational analysis revealed their potential involvement in post-transcriptional regulation of mRNA transcripts suggesting functional convergence among different small RNA classes in germ cells and zygotes.
Description18 p.-4 tab.-7 fig.
Publisher version (URL)http://www.rnajournal.org/cgi/doi/10.1261/rna.048215.114
URIhttp://hdl.handle.net/10261/113790
DOI10.1261/rna.048215.114
ISSN1355-8382
E-ISSN1469-9001
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
RNA-2015-García-López-rna.048215.114.pdf2,24 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.