English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/113343
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Distinct type I and type II toxin-antitoxin modules control Salmonella lifestyle inside eukaryotic cells

AuthorsLobato-Márquez, Damián ; Moreno-Córdoba, Inmaculada; Figueroa, Virginia; Díaz-Orejas, Ramón ; García del Portillo, Francisco
Issue Date20-Mar-2015
PublisherNature Publishing Group
CitationScientific Reports 5 (9374)
AbstractToxin-antitoxin (TA) modules contribute to the generation of non-growing cells in response to stress. These modules abound in bacterial pathogens although the bases for this profusion remain largely unknown.Using the intracellular bacterial pathogen Salmonella enterica serovar Typhimurium as a model, here we show that a selected group of TA modules impact bacterial fitness inside eukaryotic cells. We characterized in this pathogen twenty-seven TA modules, including type I and type II TA modules encoding antisense RNA and proteinaceous antitoxins, respectively. Proteomic and gene expression analyses revealed that the pathogen produces numerous toxins of TA modules inside eukaryotic cells. Among these, the toxins HokST,LdrAST, and TisBST, encoded by type I TA modules and T4ST and VapC2ST, encoded by type II TA modules,promote bacterial survival inside fibroblasts. In contrast, only VapC2ST shows that positive effect in bacterial fitness when the pathogen infects epithelial cells. These results illustrate howS. Typhimurium uses distinct type I and type II TA modules to regulate its intracellular lifestyle in varied host cell types. This function specialization might explain why the number of TA modules increased in intracellular bacterial pathogens.
Description10 p.-5 fig.-2 tab.
Publisher version (URL)http://dx.doi.org/10.1038/srep09374
URIhttp://hdl.handle.net/10261/113343
DOI10.1038/srep09374
ISSN2045-2322
E-ISSN2045-2322
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Scientific Reports_Díaz-Orejas_2015pdf.pdf1,88 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.