Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/113184
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Synergistic effect of pendant hydroxypropyl and pyrrolidine moieties randomly distributed along polymethacrylamide backbones on in vitro DNA-transfection |
Autor: | Redondo, J. A.; Velasco, Diego CSIC ORCID; Pérez-Perrino, Mónica CSIC ORCID; Reinecke, Helmut CSIC ORCID; Gallardo Ruiz, Alberto CSIC ORCID; Pandit, A.; Elvira, Carlos CSIC ORCID | Palabras clave: | HPMA Gene delivery Pyrrolidine Stimuli responsive polymers Cationic polymers Acrylics |
Fecha de publicación: | 2015 | Editor: | Elsevier | Citación: | European Journal of Pharmaceutics and Biopharmaceutics 90: 38-43 (2015) | Resumen: | In this work, the cationic monomer N-ethyl pyrrolidine methacrylamide (EPA) was copolymerized with the neutral monomer N-hydroxypropyl methacrylamide (HPMA) at different molar ratios obtaining linear random copolymers that were characterized and evaluated in vitro as non-viral gene carriers using murine Swiss 3T3 fibroblasts. The copolymers with excess or equimolar amount of EPA were able to complex DNA forming stable polyplexes with an average size between 50 and 200 nm, while the copolymers with an excess of HPMA do not. Cell viability was shown to depend on the EPA/HPMA molar ratio, exhibiting the equimolar copolymer poly (EPA-co-HPMA) 50:50 (EPA50) a full cytocompatibility, similar to the HPMA-rich systems. This copolymer EPA50 has also shown significantly higher transfection levels than the systems with other compositions and the positive controls poly l-lysine (PLL) and poly EPA (pEPA). This statistical equimolar copolymer EPA50 has unique properties related to its composition and microstructure, which allows it to complex DNA, showing an excellent biocompatibility and high transfection efficiency. | URI: | http://hdl.handle.net/10261/113184 | DOI: | 10.1016/j.ejpb.2014.11.006 | Identificadores: | doi: 10.1016/j.ejpb.2014.11.006 issn: 0939-6411 e-issn: 1873-3441 |
Aparece en las colecciones: | (ICTP) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
6
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
6
checked on 25-feb-2024
Page view(s)
337
checked on 24-abr-2024
Download(s)
182
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.