English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/112654
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Differential distribution of PDE4D splice variant mRNAs in rat brain suggests association with specific pathways and presynaptical localization

AuthorsMiró, Xavier; Pérez-Torres, Silvia; Puigdomènech, Pere ; Palacios, José M.; Mengod Los Arcos, Guadalupe
KeywordsRolipram
In situ hybridization
Area postrema
Serotonergic cells
Emesis
Depression
Issue Date9-Jul-2002
PublisherJohn Wiley & Sons
CitationSynapse 45(4): 259-269 (2002)
AbstractcAMP plays an important role as a second-messenger molecule controlling multiple cellular processes. Its hydrolysis provides an important mechanism by which cAMP levels are regulated. This is performed by a large multigene family of cyclic nucleotide phosphodiesterases (PDEs). Members of the PDE4 enzyme family are selectively inhibited by rolipram. Five different mRNA splice forms for PDE4D have been isolated. Here, we analyzed the regional distribution of the mRNAs coding for the splice variants PDE4D1, PDE4D2, PDE4D3, PDE4D4, and PDE4D5 in the rat brain by in situ hybridization histochemistry using specific radiolabeled oligonucleotides. We found that all five splice variants showed a distinct distribution pattern and, in some cases, in association with specific brain pathways. The most relevant differences were in hippocampal formation, medial habenula, basal ganglia, and area postrema, at both the regional and cellular level. The dorsal and median raphe nuclei exclusively contained PDE4D2 mRNA transcripts, probably located on serotonergic cells. PDE4D1 mRNA was expressed in some white matter cells. PDE4D1 and PDE4D2 mRNA splice forms presented a similar distribution in the area postrema, whereas for PDE4D4 and PDE4D5 the cellular distribution presented a complementary pattern. The differential expression of PDE4D mRNA splice variants in the area postrema is consistent with their possible involvement in emesis control and suggests new molecular targets for a more selective drug design. © 2002 Wiley-Liss, Inc.
Publisher version (URL)http://dx.doi.org/10.1002/syn.10100
URIhttp://hdl.handle.net/10261/112654
DOI10.1002/syn.10100
Identifiersdoi: 10.1002/syn.10100
issn: 0887-4476
Appears in Collections:(CID) Artículos
(IBMB) Artículos
(IIBB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.