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Título: | Plasmodium falciparum inhibitor-3 homolog increases protein phosphatase type 1 activity and is essential for parasitic survival |
Autor: | Fréville, Aline; Landrieu, Isabelle; García-Gimeno, María Adelaida CSIC ORCID; Kalamou, Hadidjatou; Montbarbon, Muriel; Bertin, Benjamin; Sanz, Pascual CSIC ORCID ; Werkmeister, Elisabeth; Pierrot, Christine; Khalife, Jamal | Palabras clave: | Plasmodium falciparum Protein phosphatase 1 Inhibitor-3 PP1 regulator |
Fecha de publicación: | 28-nov-2011 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 287(2):1306-1321. (2012) | Resumen: | Growing evidence indicates that the protein regulators governing protein phosphatase 1 (PP1) activity have crucial functions because their deletion drastically affects cell growth and division. PP1 has been found to be essential in Plasmodium falciparum, but little is known about its regulators. In this study, we have identified a homolog of Inhibitor-3 of PP1, named PfI3. NMR analysis shows that PfI3 belongs to the disordered protein family. High affinity interaction of PfI3 and PfPP1 is demonstrated in vitro using several methods, with an apparent dissociation constant K(D) of 100 nm. We further show that the conserved (41)KVVRW(45) motif is crucial for this interaction as the replacement of the Trp(45) by an Ala(45) severely decreases the binding to PfPP1. Surprisingly, PfI3 was unable to rescue a yeast strain deficient in I3 (Ypi1). This lack of functional orthology was supported as functional assays in vitro have revealed that PfI3, unlike yeast I3 and human I3, increases PfPP1 activity. Reverse genetic approaches suggest an essential role of PfI3 in the growth and/or survival of blood stage parasites because attempts to obtain knock-out parasites were unsuccessful, although the locus of PfI3 is accessible. The main localization of a GFP-tagged PfI3 in the nucleus of all blood stage parasites is compatible with a regulatory role of PfI3 on the activity of nuclear PfPP1. | Descripción: | 16 páginas, 9 figuras. Contiene en material suplementario: Figs. S1–S3 and Tables S1 and S2."This research was originally published in Journal of Biological Chemistry. Fréville A, Landrieu I, García-Gimeno MA, Vicogne J, Montbarbon M, Bertin B, Verger A, Kalamou H, Sanz P, Werkmeister E, Pierrot C, Khalife J. Plasmodium falciparum inhibitor-3 homolog increases protein phosphatase type 1 activity and is essential for parasitic survival. J Biol Chem. 2012 Jan 6;287(2):1306-21. © the American Society for Biochemistry and Molecular Biology" | Versión del editor: | http://dx.doi.org/10.1074/jbc.M111.276865 | URI: | http://hdl.handle.net/10261/112521 | DOI: | 10.1074/jbc.M111.276865 | ISSN: | 0021-9258 | E-ISSN: | 1083-351X |
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