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Critical role of hydrogen peroxide signaling in the sequential activation of p38 MAPK and eNOS in laminar shear stress

AuthorsBretón-Romero, Rosa ; González de Orduña, Cecilia; Romero, Natalia; Sánchez-Gómez, Francisco J. ; Álvaro, Cristina de; Porras, Almudena; Rodríguez-Pascual, Fernando ; Laranjinha, Joao; Radi, Rafael; Lamas Peláez, Santiago
Keywordsp38 MAPK
Free radicals
NADPH oxidase
Hydrogen peroxide
Endothelial nitric oxide
Laminar shear stress
Issue Date2012
CitationFree Radical Biology and Medicine 52: 1093- 1100 (2012)
AbstractLaminar shear stress (LSS) is a protective hemodynamic regulator of endothelial function and limits the development of atherosclerosis and other vascular wall diseases related to pathophysiological generation of reactive oxygen species. LSS activates several endothelial signaling responses, including the activation of MAPKs and eNOS. Here, we explored the mechanisms of activation of these key endothelial signaling pathways. Using the cone/plate model we found that LSS (12 dyn/cm 2) rapidly promotes endothelial intracellular generation of superoxide and hydrogen peroxide (H 2O 2). Physiological concentrations of H 2O 2 (flux of 0.1 nM/min and 15 οM added extracellularly) significantly activated both eNOS and p38 MAPK. Pharmacological inhibition of NADPH oxidases (NOXs) and specific knockdown of NOX4 decreased LSS-induced p38 MAPK activation. Whereas the absence of eNOS did not alter LSS-induced p38 MAPK activation, pharmacological inhibition and knockdown of p38α MAPK blocked H 2O 2- and LSS-induced eNOS phosphorylation and reduced •NO levels. We propose a model in which LSS promotes the formation of signaling levels of H 2O 2, which in turn activate p38α MAPK and then stimulate eNOS, leading to increased •NO generation and protection of endothelial function. © 2012 Elsevier Inc. All rights reserved.
Identifiersdoi: 10.1016/j.freeradbiomed.2011.12.026
issn: 0891-5849
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