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Title

Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120

AuthorsOlmedo-Verd, E. ; Muro-Pastor, Alicia M.; Flores, Enrique ; Herrero, Antonia
Issue Date2006
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 188: 6694- 6699 (2006)
AbstractThe ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P 2) and promoters that are induced upon N step-down (P1 and P3). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P3 and P1. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
URIhttp://hdl.handle.net/10261/111751
DOI10.1128/JB.00509-06
Identifiersdoi: 10.1128/JB.00509-06
issn: 0021-9193
Appears in Collections:(IBVF) Artículos
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