English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/111747
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor

AuthorsOlmedo-Verd, E. ; Flores, Enrique ; Herrero, Antonia ; Muro-Pastor, Alicia M.
Issue Date2005
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 187: 1985- 1991 (2005)
AbstractHeterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120 depends on both the global nitrogen control transcription factor NtcA and the cell differentiation regulatory protein HetR, with expression of ntcA and hetR being dependent on each other. In this study we constructed strains that constitutively express the ntcA gene leading to high levels of NtcA protein irrespective of the nitrogen source, and we analyzed the elects of such NtcA levels on heterocyst diferentiation. In the NtcA-overproducing strain, heterocyst differentiation, induction of NtcA-dependent heterocyst development genes or operons such as devBCA or the cox2 operon, and NtcA-dependent excision of the 11-kb nifD-intervening element only took place under nitrogen deficiency. Although functional heterocysts were produced in response to nitrogen step-down, the NtcA over-producing strain could not grow diazotrophically. Overexpression of ntcA in a hetR background promoted expression of devBCA in response to ammonium withdrawal and excision of the 11-kb element even in the presence of combined nitrogen. Our results show that some NtcA-dependent heterocyst-related genes can be expressed independently of HetR. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
URIhttp://hdl.handle.net/10261/111747
DOI10.1128/JB.187.6.1985-1991.2005
Identifiersdoi: 10.1128/JB.187.6.1985-1991.2005
issn: 0021-9193
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
J. Bacteriol.-2005-Olmedo-Verd-1985-91.pdf239,69 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.