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Title

Plasmid rolling-circle replication

AuthorsRuiz-Masó, José A. ; Machón, Cristina ; Bordanaba-Ruiseco, Lorena; Espinosa, Manuel ; Coll, Miquel ; Solar, Gloria del
KeywordsRolling-circle replication
Leading-strand synthesis
Lagging-strand synthesis
Plasmid
Promiscuity
Rep proteins
PcrA helicase
Issue Date20-Feb-2015
PublisherAmerican Society for Microbiology
CitationMicrobiology Spectrum. 02/2015; 3(1):PLAS-0035-2014
AbstractPlasmids are DNA entities that undergo controlled replication independent of the chromosomal DNA, a crucial step that guarantees the prevalence of the plasmid in its host.DNA replication has to cope with the incapacity of the DNA polymerases to start de novo DNA synthesis, and different replication mechanisms offer diverse solutions to this problem.Rolling-circle replication (RCR) is a mechanism adopted by certain plasmids, among other genetic elements, that represents one of the simplest initiation strategies, that is, the nicking by a replication initiator protein on one parental strand to generate the primer for leading-strand initiation and a single priming site for lagging-strand synthesis. All RCR plasmid genomes consist of a number of basic elements: leading strand initiation and control, lagging strand origin, phenotypic determinants, and mobilization, generally in that order of frequency. RCR has been mainly characterized in Gram-positive bacterial plasmids, although it has also been described in Gram-negative bacterial or archaeal plasmids. Here we aim to provide an overview of the RCR plasmids’ lifestyle, with emphasis on their characteristic traits, promiscuity, stability, utility as vectors, etc.While RCR is one of the best-characterized plasmid replication mechanisms, there are still many questions left unanswered,which will be pointed out along the way in this review.
Description51 p.-4 fig.
Publisher version (URL)http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.PLAS-0035-2014
http://dx.doi.org/10.1128/microbiolspec.PLAS-0035-2014
URIhttp://hdl.handle.net/10261/111631
DOI10.1128/microbiolspec.PLAS-0035-2014
E-ISSN2165-0497
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