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Open Access item Molecular characterization and genetic diversity of Prunus rootstocks

Authors:Bouhadida, Mariem
Casas Cendoya, Ana María
Gonzalo Pascual, María José
Arús, Pere
Moreno Sánchez, María Ángeles
Gogorcena Aoiz, Yolanda
Keywords:Cultivar identification, Genetic relationship, Peach-based rootstocks, Plums, SSR markers
Issue Date:Apr-2009
Publisher:Elsevier
Citation:Scientia Horticulturae 120 (2): 237-245 (2009)
Abstract:Twenty microsatellite primer pairs, previously developed in peach, were used to characterize and to explore genetic relationships among 44 clones, representing three groups of rootstocks defined as: (1) Peach-based rootstocks (Prunus dulcis x P. persica, P. persica x P. davidiana); (2) Myrobalan - Marianna plums (P. cerasifera, and interspecific hybrids having P. cerasifera as a parent); and (3) Slow growing plums (P. insititia, P. domestica, and P. domestica x P. spinosa). Eighteen SSR markers, from the 20 initially used, were able to amplify polymorphic products for the Peach-based rootstocks and 13 common markers gave also polymorphism for the Myrobalan-Marianna and Slow growing plums groups. The Dice coefficient of similarity was calculated between all pairs of accessions and their genetic similarity represented by a principal coordinate analysis. The genetic diversity detected among the 44 clones studied divided them in three groups, which are in agreement with their current taxonomic classification and their morphological characteristics. A set of three microsatellites (BPPCT001, CPPCT022 and UDP98-407) can distinguish between all the clones analyzed. The analysis within groups reveal another two sets of three SSR to distinguish between the clones from the peach based rootstocks and the myrobalan-Marianna plums respectively and only a single SSR is needed to distinguish within the clones from the Slow growing plums group. These results demonstrate the high potential of the SSR analysis for peach rootstock identification and studies of diversity in Prunus species.
Description:The definitive version is available at: http://www.sciencedirect.com/science/journal/03044238
Publisher version (URL):http://dx.doi.org/10.1016/j.scienta.2008.11.015
URI:http://hdl.handle.net/10261/11160
ISSN:0304-4238
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