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Título: | Sensitive monoclonal antibody-based immunoassays for kresoxim-methyl analysis in QuEChERS-based food extracts |
Autor: | Mercader Badia, Josep Vicent CSIC ORCID; López Moreno, Rosario; Esteve Turrillas, Francesc A. CSIC; Agulló, Consuelo; Abad Somovilla, Antonio; Abad Fuentes, Antonio CSIC ORCID | Palabras clave: | QuEChERS Deming regression Residues Strobilurins Rapid methods Competitive ELISA |
Fecha de publicación: | 2014 | Editor: | American Chemical Society | Citación: | Journal of agricultural and food chemistry 62: 2816- 2821 (2014) | Resumen: | Kresoxim-methyl is nowadays widely used to combat a diversity of common diseases affecting high-value crops. In this article, we report the development and characterization of two novel immunoassays for the analysis of this pioneer strobilurin fungicide, and for the first time, a validation study with food samples was performed. A direct and an indirect competitive immunoassay based on a new anti-kresoxim-methyl monoclonal antibody were developed for sensitive and specific chemical analysis. Optimized assays showed limits of detection of 0.1 μg/L. Fruit and vegetable samples were extracted with acetonitrile by the QuEChERS procedure and analyzed by the developed immunoassays after a simple dilution in buffer, affording limits of quantification below US and European maximum residue limits. Immunochemical results of samples from kresoxim-methyl-sprayed strawberry fields demonstrated good statistical agreement with gas chromatography coupled to mass spectrometry as reference technique. © 2014 American Chemical Society. | URI: | http://hdl.handle.net/10261/110862 | DOI: | 10.1021/jf500287t | Identificadores: | doi: 10.1021/jf500287t issn: 1520-5118 |
Aparece en las colecciones: | (IATA) Artículos |
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