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Título: | DNA labeling in vivo: Quantification of epidermal stem cell chromatin content in whole mouse hair follicles using Fiji image processing software |
Autor: | Carrasco, Elisa CSIC ORCID; Calvo, María I.; Espada, Jesús CSIC ORCID | Palabras clave: | Label-retaining cells Epidermal stem cells DNA labeling Bromo-deoxyuridine (BrdU) Hair follicle Fiji software |
Fecha de publicación: | 2014 | Editor: | Humana Press Springer Nature |
Citación: | Functional Analysis of DNA and Chromatin (Cap.7): 79-88 (2014) | Serie: | Methods in Molecular Biology 1094 |
Resumen: | DNA labeling in vivo using nucleoside analogues is a current experimental approach to determine cell proliferation rates in cell cultures and tissues. It has also been successfully used to localize adult stem cell niches through the identification of nucleoside label-retaining cells (LRC) in long-term experiments. A major hindrance of this methodology relies on the selection of adequate procedures to quantify the nucleoside analogue content from image data files. Here we propose a simple procedure using Fiji image processing software to accurately calculate nucleoside analogue retaining chromatin/total chromatin (LRC/DAPI) signal ratios in the well-known mouse hair follicle stem cell niche. | Versión del editor: | http://dx.doi.org/10.1007/978-1-62703-706-8_7 | URI: | http://hdl.handle.net/10261/110700 | DOI: | 10.1007/978-1-62703-706-8_7 | ISBN: | 978-1-62703-705-1 |
Aparece en las colecciones: | (IIBM) Libros y partes de libros |
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DNA Labeling in Vivo.pdf | 566,47 kB | Adobe PDF | Visualizar/Abrir |
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