Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/110250
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Disulfide linkage Raman markers: A reconsideration attempt |
Autor: | Hernández, B.; Pflüger, Fernando; López-Tobar, Eduardo CSIC; Kruglik, Sergei G.; García-Ramos, José Vicente CSIC ORCID; Sánchez-Cortés, Santiago CSIC ORCID; Ghomi, Mahmoud | Palabras clave: | Density functional theory Multiconformational analysis Cystine Diethyl disulfide Dimethyl disulfide Peptides Disulfide linkage Raman markers Proteins |
Fecha de publicación: | 2014 | Editor: | John Wiley & Sons | Citación: | Journal of Raman Spectroscopy 45: 657- 664 (2014) | Resumen: | During the last decades, Raman spectroscopy has been routinely used for probing the conformational features of disulfide linkages in peptides and proteins. However, the interpretation of disulfide Raman markers is currently performed by a simple rule derived from the earliest observations on dialkyl disulfides. More precisely, this rule consists of the following: (1) in analyzing the Raman bands in the 550-500 cm-1 region ascribed to disulfide bond stretch motion, namely, ν(S-S), and (2) assigning the three types of Raman markers observed at ~500, ~520, and ~540 cm-1 to three families of rotamers defined along the three successive bonds of the -C-S-S-C- moiety, referred to as ggg, ggt, and tgt. In this report, we attempt to show that an accurate analysis of disulfide vibrational features needs the use of the five torsion angles (χ1, χ2, χ3, χ2', and χ1') along the five successive bonds joining the two α-carbon atoms in the cystine (Cys-Cys) unit. The present work is inspired by the disulfide conformational investigations performed by a statistical scan of numerous protein crystal and nuclear magnetic resonance data, taking into account the handedness (right and left) of a disulfide bridge, its spatial shape (Staple, Hook, and Spiral), as well as the signs of the two extreme torsion angles χ1 and χ1'. It appears that the combined use of the old and recent conformational notations allows a more accurate structural and vibrational analysis of disulfide linkage. Copyright © 2014 John Wiley & Sons, Ltd. It has been shown that the old notations (ggg, ggt, and tgt) are insufficient for analyzing the conformational and vibrational features of disulfide linkages in peptides and proteins. This failure can be improved by considering the new topological notations based on the signs of the five torsion angles in going from one α-carbon to the other in the cystine unit. Copyright © 2014 John Wiley & Sons, Ltd. | URI: | http://hdl.handle.net/10261/110250 | DOI: | 10.1002/jrs.4521 | Identificadores: | doi: 10.1002/jrs.4521 issn: 1097-4555 |
Aparece en las colecciones: | (CFMAC-IEM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
41
checked on 25-mar-2024
WEB OF SCIENCETM
Citations
38
checked on 17-feb-2024
Page view(s)
243
checked on 28-mar-2024
Download(s)
109
checked on 28-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.