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Improving the WFD purposes by the incorporation of ecotoxicity tests

AuthorsRoig, Neus; Sierra, Jordi; Nadal, Martí; Moreno-Garrido, Ignacio ; Nieto, Elena ; Hampel, Miriam ; Blasco, Julián ; Schuhmacher, Marta; Domingo, José L.
Issue DateNov-2013
Citation4th SCARCE International Conference (2013)
AbstractThe approval of the European Water Framework Dir ective (WFD) supposed a big step regarding aquatic ecosystems protection. According to this Directive, assessment of ecological status is based on three quality elements: biological, physicoc hemical and hydromorphological, but ecotoxicological status is still not included. Some studies have observed that biol ogical status is not always in coherence with physicochemical status, maybe due to the adaptati on mechanisms of aquatic organisms under chronic chemical exposure. In these situations, ecotoxicity t ests could be useful to obtain a better characterisation of these specific ecosystems. The general aim of this work is to add a battery of ecotoxicity tests to the current analyses defined by WFD in order to obtain a better ecological characteriza tion of freshwater systems. The specific aims of this work are: (1) to compare the effectiveness and viability of differen t ecotoxicity tests performed with freshwater sediments (directly and with pore water) ta king as target organisms different aquatic species, and (2) to evaluate the relationship between stream pollutants concentrations (organic pollutants and metals), biological and hydromorphological status and sediments ecotoxicity. For this purpose, thirteen sampling sites within the Ebro river watershed were selected. Data about priority pollutants in water, sediment and fish as well as biological and hydromor phological status of each sampling point will be achieved. Moreover, in each sampling reach, composite samples of sediment were collected by using a Van Veen grab. Sediment samples were stored at 4ºC prior to the ecotoxicity analyses
The ecotoxicity of pore water was evaluated by different bioassays ( Vibrio fischeri, Pseudokirshneriella subcapitata and Daphnia magna ) while the ecotoxicity of wh ole sediment was evaluated in Vibrio fischeri, Nitzschia palea and Chironomus riparius In addition, the concentration of total heavy metals and metal bioavailability was calculated by a sequential extraction according to the Community Bureau of Reference (BCR) method. To distinguish the potentially toxic fraction associated to heavy metals burden of sediments, an analysis of acid-volatile sulphide (AVS) and simultaneously extracted metals (SEM) was performed. Complementary sediment variables as humi dity, porosity, percentages of fines (<63 μm) organic carbon and organic matter were determined. This study expect to demonstrate that the integr ation of chemical, biological and ecotoxicological analyses could be crucial to unde rstand the hazard of pollutants in aquatic ecosystems, especially, in freshwater sediments. Future research in this area is needed in order to obtain more data and be able to establish a tree decision of freshwater analyses ev aluation. The poster will present the methodology purposed for this study as well as the first prelim inary results obtained from ecotoxicity tests.
DescriptionTrabajo presentado en la 4th SCARCE International Conference (Towards a better understanding of the links between stressors, hazard assessment and ecosystem services under water scarcity), celebrada en Cádiz el 25 y 26 de noviembre de 2013.
Appears in Collections:(ICMAN) Comunicaciones congresos
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