English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/110001
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Early neural cell death is an extensive, dynamic process in the embryonic chick and mouse retina

AuthorsChavarría, Teresa; Baleriola, Jimena ; Mayordomo, Raquel; Pablo, Flora de ; De la Rosa, Enrique J.
Issue Date2013
PublisherHindawi Publishing Corporation
CitationThe Scientific World Journal (2013),ID 627240
AbstractOrchestrated proliferation, differentiation, and cell death contribute to the generation of the complex cytoarchitecture of the central nervous system, including that of the neuroretina. However, few studies have comprehensively compared the spatiotemporal patterns of these 3 processes, or their relative magnitudes. We performed a parallel study in embryonic chick and mouse retinas, focusing on the period during which the first neurons, the retinal ganglion cells (RGCs), are generated. The combination of in vivo BrdU incorporation, immunolabeling of retinal whole mounts for BrdU and for the neuronal markers Islet1/2 and βIII-tubulin, and TUNEL allowed for precise cell scoring and determination the spatiotemporal patterns of cell proliferation, differentiation, and death. As predicted, proliferation preceded differentiation. Cell death and differentiation overlapped to a considerable extent, although the magnitude of cell death exceeded that of neuronal differentiation. Precise quantification of the population of recently born RGCs, identified by BrdU and βIII-tubulin double labeling, combined with cell death inhibition using a pan-caspase inhibitor, revealed that apoptosis decreased this population by half shortly after birth. Taken together, our findings provide important insight into the relevance of cell death in neurogenesis.
Description9 p.-4 fig.
Publisher version (URL)http://dx.doi.org/10.1155/2013/627240
URIhttp://hdl.handle.net/10261/110001
DOI10.1155/2013/627240
ISSN2356-6140
E-ISSN1537-744X
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
The Scientific World Journal, 2013,Flora de Pablo.pdf5,52 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.