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Title

A multiple sample immunoblotting system (MSIS) for the intrinsic detection of antinuclear autoantibodies

AuthorsNavarro, Estanislau; Bach-Elias, Montse ; Durán, Núria; Puigdomènech, Pere ; Palau, Jaume
KeywordsAnti-RNP
Autoimmune disease
Anti-Sm
Anti-SS/B
Immunoblotting
Antinuclear antibody
Issue Date11-Jul-1986
PublisherElsevier
CitationJournal of Immunological Methods 91(1): 75-81 (1986)
AbstractA multiple sample immunoblotting system (MSIS) is described. The MSIS permits the detection and classification of antinuclear antibodies (ANA) according to the pattern of antigenic polypeptides recognized in four different extracts: calf thymus whole tissue, nuclear extract, and two ammonium sulphate fractions of the nuclear extract. The procedure permits the classification of anti-RNP, anti-SS/B and anti-Sm, and the detection of new ANAs in sera from SLE patients. The reaction patterns presented are specific and ambiguous, and make the use of validated control sera unnecessary.
URIhttp://hdl.handle.net/10261/109162
DOI10.1016/0022-1759(86)90104-3
Identifiersdoi: 10.1016/0022-1759(86)90104-3
issn: 0022-1759
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