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Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus

AutorGuil, Sonia ; Iglesia, Núria de la; Fernández-Larrea, Juan; Cifuentes, Daniel; Ferrer, Juan Carlos; Guinovart, Joan J.; Bach-Elias, Montse
Fecha de publicación1-sep-2003
EditorAmerican Association for Cancer Research
CitaciónCancer Research 63: 5178-5187 (2003)
ResumenWe characterized a novel protein of the Ras family, p19 (H-RasIDX). The c-H-ras proto-oncogene undergoes alternative splicing of the exon termed IDX. We show that the alternative p19 mRNA is stable and as abundant as p21 (p21 H-Ras4A) mRNA in all of the human tissues and cell lines tested. IDX is spliced into stable mRNA in different mammalian species, which present a high degree of nucleotide conservation. Both the endogenous and the transiently expressed p19 protein are detected in COS-1 and HeLa cells and show nuclear diffuse and speckled patterns as well as cytoplasmic localization. In yeast two-hybrid assays, p19 did not interact with two known p21 effectors, Raf1 and Rin1, but was shown to interact with RACK1, a scaffolding protein that promotes multiprotein complexes in different signaling pathways. This observation suggests that p19 and p21 play differential and complementary roles in the cell.
Identificadoresissn: 0008-5472
issn: 1538-7445
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